Detailed information of TA system TA208685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 354312..354789
• Replicon: chromosome
• Accession: NZ_CP077932
• Organism: Staphylococcus aureus strain 194

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: KU515_RS01865
• Protein ID: WP_000052076.1
• Coordinates: 354312..354500 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: -
• Locus tag: KU515_RS01870
• Protein ID: WP_225798476.1
• Coordinates: 354529..354789 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU515_RS01835 (350302)	350302..350823	+	522	WP_000824479.1	hypothetical protein	-
KU515_RS01840 (351164)	351164..351850	+	687	WP_001622483.1	thermonuclease family protein	-
KU515_RS01845 (352207)	352207..352407	+	201	WP_001059082.1	cold-shock protein	-
KU515_RS01850 (352904)	352904..353122	-	219	WP_000005884.1	hypothetical protein	-
KU515_RS01855 (353195)	353195..353467	-	273	WP_001004400.1	hypothetical protein	-
KU515_RS01860 (353555)	353555..354124	-	570	WP_001106662.1	lipoprotein N-acylation protein LnsA	-
KU515_RS01865 (354312)	354312..354500	-	189	WP_000052076.1	hypothetical protein	Toxin
KU515_RS01870 (354529)	354529..354789	-	261	WP_225798476.1	hypothetical protein	Antitoxin
KU515_RS01875 (355130)	355130..355333	+	204	WP_000145160.1	sterile alpha motif-like domain-containing protein	-
KU515_RS01880 (355330)	355330..355566	-	237	WP_000368687.1	hypothetical protein	-
KU515_RS01885 (355790)	355790..356371	+	582	WP_001140696.1	histidine phosphatase family protein	-
KU515_RS01890 (356444)	356444..357061	-	618	WP_000255550.1	LysE/ArgO family amino acid transporter	-
KU515_RS01895 (357217)	357217..357711	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
KU515_RS01900 (358109)	358109..358531	-	423	WP_000974451.1	organic hydroperoxide resistance protein	-
KU515_RS01905 (358679)	358679..359395	+	717	WP_000150033.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sec / sell / vWbp	275814..360017	84203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T208685 WP_000052076.1 NZ_CP077932:c354500-354312 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T208685 NZ_CP103705:163994-164299 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9950.13 Da
• Isoelectric Point: 4.0624

>AT208685 WP_225798476.1 NZ_CP077932:c354789-354529 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVPFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT208685 NZ_CP103705:163774-163992 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18