Detailed information of TA system TA208684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 324980..325509
• Replicon: chromosome
• Accession: NZ_CP077932
• Organism: Staphylococcus aureus strain 194

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: KU515_RS01700
• Protein ID: WP_001103939.1
• Coordinates: 325192..325509 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: KU515_RS01695
• Protein ID: WP_001058494.1
• Coordinates: 324980..325189 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU515_RS01655 (320401)	320401..320577	+	177	WP_168240022.1	hypothetical protein	-
KU515_RS01660 (320574)	320574..321188	+	615	WP_029550584.1	hypothetical protein	-
KU515_RS01665 (321627)	321627..321830	+	204	WP_017804629.1	hypothetical protein	-
KU515_RS01670 (322337)	322337..323443	-	1107	WP_000149511.1	tyrosine-type recombinase/integrase	-
KU515_RS01675 (323433)	323433..324236	-	804	WP_000358990.1	helix-turn-helix transcriptional regulator	-
KU515_RS01680 (324372)	324372..324575	+	204	WP_001045296.1	transcriptional regulator	-
KU515_RS01685 (324611)	324611..324829	+	219	WP_000163544.1	helix-turn-helix transcriptional regulator	-
KU515_RS01690 (324841)	324841..324987	+	147	WP_000784885.1	hypothetical protein	-
KU515_RS01695 (324980)	324980..325189	+	210	WP_001058494.1	hypothetical protein	Antitoxin
KU515_RS01700 (325192)	325192..325509	+	318	WP_001103939.1	DUF1474 family protein	Toxin
KU515_RS01705 (325573)	325573..326442	+	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
KU515_RS01710 (326459)	326459..327916	+	1458	WP_000390453.1	virulence-associated E family protein	-
KU515_RS01715 (328217)	328217..328579	+	363	WP_001039170.1	hypothetical protein	-
KU515_RS01720 (328581)	328581..328865	+	285	WP_000998185.1	hypothetical protein	-
KU515_RS01725 (328862)	328862..329503	+	642	WP_001019808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sec / sell / vWbp	275814..360017	84203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T208684 WP_001103939.1 NZ_CP077932:325192-325509 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T208684 NZ_CP103705:c162987-162571 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT208684 WP_001058494.1 NZ_CP077932:324980-325189 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT208684 NZ_CP103705:c163214-162984 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IY59