Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4135032..4135672 Replicon chromosome
Accession NC_004547
Organism Pectobacterium atrosepticum SCRI1043

Toxin (Protein)


Gene name vagD Uniprot ID Q6D0W3
Locus tag ECA_RS18200 Protein ID WP_011095186.1
Coordinates 4135032..4135448 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6D0W2
Locus tag ECA_RS18205 Protein ID WP_011095187.1
Coordinates 4135445..4135672 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECA_RS18175 4130795..4132126 + 1332 WP_011095181.1 type I-F CRISPR-associated protein Csy1 -
ECA_RS18180 4132123..4133055 + 933 WP_011095182.1 type I-F CRISPR-associated protein Csy2 -
ECA_RS18185 4133069..4134082 + 1014 WP_011095183.1 type I-F CRISPR-associated protein Csy3 -
ECA_RS18190 4134092..4134646 + 555 WP_011095184.1 type I-F CRISPR-associated endoribonuclease Cas6/Csy4 -
ECA_RS18200 4135032..4135448 - 417 WP_011095186.1 type II toxin-antitoxin system VapC family toxin Toxin
ECA_RS18205 4135445..4135672 - 228 WP_011095187.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECA_RS18210 4136630..4137049 - 420 WP_011095188.1 secondary thiamine-phosphate synthase enzyme YjbQ -
ECA_RS18215 4137067..4139901 - 2835 WP_011095189.1 excinuclease ABC subunit UvrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15299.98 Da        Isoelectric Point: 8.7720

>T20868 WP_011095186.1 NC_004547:c4135448-4135032 [Pectobacterium atrosepticum SCRI1043]
VSRLFMLDTNICSFIMREHPSAVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMLLVDAFCRRLDAILPWDSA
AVDATTKIKIALAAAVTPIGPNDTAIAGHAISAKAILVTNNLREFERVPELILEDWVK

Download         Length: 417 bp

>T20868 NC_004547:c4135448-4135032 [Pectobacterium atrosepticum SCRI1043]
GTGAGCCGTTTGTTCATGCTTGATACTAATATCTGTTCTTTCATTATGCGTGAACACCCGTCTGCGGTGATTGAAAAGTT
GCAGCAATGCACACTGCGACGAGATCGAATCGTGGTATCAGCTATCACGTACGCTGAAATGCGTTTTGGGGCGGTTGGCA
AAAAAGCTTCGCCGCGCCATATGCTGCTGGTTGATGCTTTCTGCCGCCGACTTGATGCGATATTACCTTGGGATAGCGCA
GCGGTAGACGCCACCACAAAAATTAAAATTGCACTAGCTGCAGCTGTCACACCTATTGGTCCAAACGATACGGCCATCGC
TGGTCATGCTATCAGCGCTAAGGCCATACTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGAACTGATACTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8894.05 Da        Isoelectric Point: 4.5216

>AT20868 WP_011095187.1 NC_004547:c4135672-4135445 [Pectobacterium atrosepticum SCRI1043]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEERADADFMTERQDVVEEGRFEL

Download         Length: 228 bp

>AT20868 NC_004547:c4135672-4135445 [Pectobacterium atrosepticum SCRI1043]
ATGCGTATTGTCTCAGTATTTAAAAATGGTAATAACCGTGCTATTCGCTTGCCGCGTGATATGGACTTTGAAGGCGTCAA
TGAACTGGAGATCGTGCGTGAAGGGGATACCATCATGTTGCGCCCAGTCAAACCCAATTGGCATTCTTTTCTGCAAGAAG
AGCGTGCTGACGCAGACTTTATGACAGAACGTCAGGATGTAGTCGAAGAGGGGAGATTCGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6D0W3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J5XN10

References