Detailed information of TA system TA208677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2472765..2473294
• Replicon: chromosome
• Accession: NZ_CP077931
• Organism: Staphylococcus aureus strain 195

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU526_RS12030
• Protein ID: WP_000621175.1
• Coordinates: 2472932..2473294 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU526_RS12025
• Protein ID: WP_000948331.1
• Coordinates: 2472765..2472935 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU526_RS11995 (2467802)	2467802..2468362	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
KU526_RS12000 (2468570)	2468570..2469049	+	480	WP_001287077.1	hypothetical protein	-
KU526_RS12005 (2469042)	2469042..2470625	+	1584	WP_001294637.1	PH domain-containing protein	-
KU526_RS12010 (2470612)	2470612..2471103	+	492	WP_001205907.1	PH domain-containing protein	-
KU526_RS12015 (2471107)	2471107..2471466	+	360	WP_000581199.1	holo-ACP synthase	-
KU526_RS12020 (2471532)	2471532..2472680	+	1149	WP_001281140.1	alanine racemase	-
KU526_RS12025 (2472765)	2472765..2472935	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU526_RS12030 (2472932)	2472932..2473294	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU526_RS12035 (2473643)	2473643..2474644	+	1002	WP_031928242.1	PP2C family protein-serine/threonine phosphatase	-
KU526_RS12040 (2474763)	2474763..2475089	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU526_RS12045 (2475091)	2475091..2475570	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU526_RS12050 (2475545)	2475545..2476315	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208677 WP_000621175.1 NZ_CP077931:2472932-2473294 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208677 NZ_CP103704:c2864384-2864001 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208677 WP_000948331.1 NZ_CP077931:2472765-2472935 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208677 NZ_CP103704:c2864845-2864465 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1