Detailed information of TA system TA020867

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3196485..3197210
• Replicon: chromosome
• Accession: NC_004547
• Organism: Pectobacterium atrosepticum SCRI1043

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: Q6D391
• Locus tag: ECA_RS14000
• Protein ID: WP_011094386.1
• Coordinates: 3196485..3196805 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: ECA_RS14005
• Protein ID: WP_043878070.1
• Coordinates: 3196875..3197210 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECA_RS13970	3191688..3191906	+	219	WP_011094380.1	KTSC domain-containing protein	-
ECA_RS13975	3192250..3193197	-	948	WP_011094381.1	LysR family transcriptional regulator	-
ECA_RS13980	3193309..3194337	+	1029	WP_011094382.1	subclass B3 metallo-beta-lactamase CAR-1	-
ECA_RS13985	3194782..3195210	-	429	WP_011094383.1	tRNA(fMet)-specific endonuclease VapC	-
ECA_RS13990	3195210..3195440	-	231	WP_011094384.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ECA_RS13995	3195534..3196367	-	834	WP_011094385.1	DUF4942 domain-containing protein	-
ECA_RS14000	3196485..3196805	-	321	WP_011094386.1	TA system toxin CbtA family protein	Toxin
ECA_RS14005	3196875..3197210	-	336	WP_043878070.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECA_RS14010	3197249..3197722	-	474	WP_011094388.1	DNA repair protein RadC	-
ECA_RS14015	3197845..3198195	-	351	WP_011094389.1	hypothetical protein	-
ECA_RS14020	3198253..3199086	-	834	WP_011094390.1	hypothetical protein	-
ECA_RS23035	3199233..3199391	-	159	WP_157863162.1	hypothetical protein	-
ECA_RS14035	3199575..3200462	-	888	WP_011094393.1	50S ribosome-binding GTPase	-
ECA_RS14040	3200558..3201589	-	1032	WP_011094394.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12143.91 Da
• Isoelectric Point: 4.8046

>T20867 WP_011094386.1 NC_004547:c3196805-3196485 [Pectobacterium atrosepticum SCRI1043]
MQTSPAIPRREDNPCPSPIAAWQQLMTYLLEKHYGLMLNDTPFCEENVIQEHIDAGITLVNAVNFLVEKYELVRIDRDGF
NWQEQSPFLTAVDVLRARRATGLLKA

Nucleotide

• Length: 321 bp

>T20867 NC_004547:c3196805-3196485 [Pectobacterium atrosepticum SCRI1043]
ATGCAAACATCACCAGCAATCCCTCGACGGGAGGATAACCCCTGTCCGTCACCCATAGCCGCTTGGCAGCAACTTATGAC
CTACCTGCTGGAAAAGCACTACGGCCTGATGCTGAATGACACACCGTTCTGCGAGGAAAACGTGATTCAGGAACATATCG
ATGCTGGCATCACGTTGGTCAATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACCGCGACGGTTTT
AACTGGCAGGAGCAATCACCGTTTCTCACTGCCGTTGATGTTCTCCGTGCTAGACGCGCAACAGGTTTACTTAAGGCATA
A

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12471.17 Da
• Isoelectric Point: 7.3241

>AT20867 WP_043878070.1 NC_004547:c3197210-3196875 [Pectobacterium atrosepticum SCRI1043]
MPSSDTPEWGLKCAITPRFGARLVQEGNRLHYLADRASVVGTFSKTEARHLEHVFPELIKQLEHKLRASELNPRQQKCVT
LHCNEWTCEADTLGSVGYVYIAIYPSSAATQ

Nucleotide

• Length: 336 bp

>AT20867 NC_004547:c3197210-3196875 [Pectobacterium atrosepticum SCRI1043]
ATGCCATCATCGGATACCCCTGAATGGGGACTAAAATGTGCCATCACTCCGCGATTCGGAGCCAGGCTGGTTCAGGAAGG
CAACCGTCTGCACTATCTGGCAGACCGGGCCAGCGTTGTCGGTACGTTCAGTAAAACAGAAGCCCGTCATCTGGAACACG
TCTTCCCTGAGCTGATTAAGCAGTTGGAGCATAAACTACGCGCGAGTGAACTGAATCCCCGTCAGCAGAAGTGCGTCACG
TTGCATTGCAACGAATGGACCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTGTATATTGCTATTTATCCCTCTTC
AGCGGCAACACAATAA

Structures

Toxin

• AlphaFold DB: Q6D391