Detailed information of TA system TA208663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 176284..176813
• Replicon: chromosome
• Accession: NZ_CP077928
• Organism: Staphylococcus aureus strain 196

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU511_RS00930
• Protein ID: WP_000621175.1
• Coordinates: 176451..176813 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU511_RS00925
• Protein ID: WP_000948331.1
• Coordinates: 176284..176454 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU511_RS00895 (171321)	171321..171881	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
KU511_RS00900 (172089)	172089..172568	+	480	WP_001287077.1	hypothetical protein	-
KU511_RS00905 (172561)	172561..174144	+	1584	WP_001294637.1	PH domain-containing protein	-
KU511_RS00910 (174131)	174131..174622	+	492	WP_001205907.1	PH domain-containing protein	-
KU511_RS00915 (174626)	174626..174985	+	360	WP_000581199.1	holo-ACP synthase	-
KU511_RS00920 (175051)	175051..176199	+	1149	WP_001281140.1	alanine racemase	-
KU511_RS00925 (176284)	176284..176454	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU511_RS00930 (176451)	176451..176813	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU511_RS00935 (177162)	177162..178163	+	1002	WP_031928242.1	PP2C family protein-serine/threonine phosphatase	-
KU511_RS00940 (178282)	178282..178608	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU511_RS00945 (178610)	178610..179089	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU511_RS00950 (179064)	179064..179834	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208663 WP_000621175.1 NZ_CP077928:176451-176813 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208663 NZ_CP103700:53928-54410 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208663 WP_000948331.1 NZ_CP077928:176284-176454 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208663 NZ_CP103700:53674-53940 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1