Detailed information of TA system TA020866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3194782..3195440
• Replicon: chromosome
• Accession: NC_004547
• Organism: Pectobacterium atrosepticum SCRI1043

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ECA_RS13985
• Protein ID: WP_011094383.1
• Coordinates: 3194782..3195210 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6D393
• Locus tag: ECA_RS13990
• Protein ID: WP_011094384.1
• Coordinates: 3195210..3195440 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECA_RS23025	3190822..3191271	-	450	WP_086935246.1	DUF2946 domain-containing protein	-
ECA_RS13970	3191688..3191906	+	219	WP_011094380.1	KTSC domain-containing protein	-
ECA_RS13975	3192250..3193197	-	948	WP_011094381.1	LysR family transcriptional regulator	-
ECA_RS13980	3193309..3194337	+	1029	WP_011094382.1	subclass B3 metallo-beta-lactamase CAR-1	-
ECA_RS13985	3194782..3195210	-	429	WP_011094383.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ECA_RS13990	3195210..3195440	-	231	WP_011094384.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ECA_RS13995	3195534..3196367	-	834	WP_011094385.1	DUF4942 domain-containing protein	-
ECA_RS14000	3196485..3196805	-	321	WP_011094386.1	TA system toxin CbtA family protein	-
ECA_RS14005	3196875..3197210	-	336	WP_043878070.1	type IV toxin-antitoxin system YeeU family antitoxin	-
ECA_RS14010	3197249..3197722	-	474	WP_011094388.1	DNA repair protein RadC	-
ECA_RS14015	3197845..3198195	-	351	WP_011094389.1	hypothetical protein	-
ECA_RS14020	3198253..3199086	-	834	WP_011094390.1	hypothetical protein	-
ECA_RS23035	3199233..3199391	-	159	WP_157863162.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15922.15 Da
• Isoelectric Point: 7.2740

>T20866 WP_011094383.1 NC_004547:c3195210-3194782 [Pectobacterium atrosepticum SCRI1043]
MLKYLLDTNICIYTIKNKPQEVRDAFYRHYGQFAISSITLMELIYGAEKSVNPEKNLAVIEGFSARMEVQNYGFDAAVHT
GQIRAELAKQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVSRQADNSQSL

Nucleotide

• Length: 429 bp

>T20866 NC_004547:c3195210-3194782 [Pectobacterium atrosepticum SCRI1043]
ATGCTTAAATATCTGCTCGATACCAATATCTGTATTTACACCATCAAGAACAAACCGCAGGAAGTCAGGGACGCTTTCTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACGTTGATGGAACTGATCTATGGTGCGGAGAAATCGGTAAATCCAG
AGAAGAATCTCGCCGTGATTGAAGGGTTTTCTGCACGTATGGAAGTTCAAAACTACGGTTTTGACGCTGCTGTACATACC
GGGCAAATTCGTGCCGAGCTGGCAAAACAAGGGACACCCATCGGTCCGTATGATGCCATGCTAGCGGGTCATGCTCGGTC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAATTTGAGCGTGTGCCTGGGCTACGTGTGGAAAACTGGGTCAGTA
GACAGGCTGACAATAGCCAGTCTTTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8719.85 Da
• Isoelectric Point: 4.4091

>AT20866 WP_011094384.1 NC_004547:c3195440-3195210 [Pectobacterium atrosepticum SCRI1043]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEEQGVTPDFMIIREQPDDQIREDF

Nucleotide

• Length: 231 bp

>AT20866 NC_004547:c3195440-3195210 [Pectobacterium atrosepticum SCRI1043]
ATGGAAAAAACTACAGTATTCAAAAGCAATCGCAGTCAGGCGGTACGGCTCCCTAAAGCCGTTGCACTGCCCGATGATGT
GAAGCAGGTTGATATCGTTGCGATTGGTCGTACACGTATTATTACTCCGGCAGGGGAAAGCTGGGACAGTTGGTTCGAAG
AACAAGGAGTAACACCTGACTTTATGATCATCAGAGAACAACCTGATGATCAGATCAGGGAAGATTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6D393