Detailed information of TA system TA208653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1239609..1240138
• Replicon: chromosome
• Accession: NZ_CP077926
• Organism: Staphylococcus aureus strain 197

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU504_RS05970
• Protein ID: WP_000621175.1
• Coordinates: 1239776..1240138 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU504_RS05965
• Protein ID: WP_000948331.1
• Coordinates: 1239609..1239779 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU504_RS05935 (1234646)	1234646..1235206	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
KU504_RS05940 (1235414)	1235414..1235893	+	480	WP_001287077.1	hypothetical protein	-
KU504_RS05945 (1235886)	1235886..1237469	+	1584	WP_001294637.1	PH domain-containing protein	-
KU504_RS05950 (1237456)	1237456..1237947	+	492	WP_001205907.1	PH domain-containing protein	-
KU504_RS05955 (1237951)	1237951..1238310	+	360	WP_000581199.1	holo-ACP synthase	-
KU504_RS05960 (1238376)	1238376..1239524	+	1149	WP_001281140.1	alanine racemase	-
KU504_RS05965 (1239609)	1239609..1239779	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU504_RS05970 (1239776)	1239776..1240138	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU504_RS05975 (1240487)	1240487..1241488	+	1002	WP_031928242.1	PP2C family protein-serine/threonine phosphatase	-
KU504_RS05980 (1241607)	1241607..1241933	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU504_RS05985 (1241935)	1241935..1242414	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU504_RS05990 (1242389)	1242389..1243159	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208653 WP_000621175.1 NZ_CP077926:1239776-1240138 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208653 NZ_CP103699:794519-794929 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208653 WP_000948331.1 NZ_CP077926:1239609-1239779 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208653 NZ_CP103699:794272-794538 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1