Detailed information of TA system TA208650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2187016..2187545
• Replicon: chromosome
• Accession: NZ_CP077925
• Organism: Staphylococcus aureus strain 198

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU531_RS10790
• Protein ID: WP_000621175.1
• Coordinates: 2187016..2187378 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU531_RS10795
• Protein ID: WP_000948331.1
• Coordinates: 2187375..2187545 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU531_RS10770 (2183995)	2183995..2184765	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU531_RS10775 (2184740)	2184740..2185219	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU531_RS10780 (2185221)	2185221..2185547	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU531_RS10785 (2185666)	2185666..2186667	-	1002	WP_031928242.1	PP2C family protein-serine/threonine phosphatase	-
KU531_RS10790 (2187016)	2187016..2187378	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU531_RS10795 (2187375)	2187375..2187545	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU531_RS10800 (2187630)	2187630..2188778	-	1149	WP_001281140.1	alanine racemase	-
KU531_RS10805 (2188844)	2188844..2189203	-	360	WP_000581199.1	holo-ACP synthase	-
KU531_RS10810 (2189207)	2189207..2189698	-	492	WP_001205907.1	PH domain-containing protein	-
KU531_RS10815 (2189685)	2189685..2191268	-	1584	WP_001294637.1	PH domain-containing protein	-
KU531_RS10820 (2191261)	2191261..2191740	-	480	WP_001287077.1	hypothetical protein	-
KU531_RS10825 (2191948)	2191948..2192508	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208650 WP_000621175.1 NZ_CP077925:c2187378-2187016 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208650 NZ_CP103698:53912-54394 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208650 WP_000948331.1 NZ_CP077925:c2187545-2187375 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208650 NZ_CP103698:53658-53924 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1