Detailed information of TA system TA020865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3144548..3145458
• Replicon: chromosome
• Accession: NC_004547
• Organism: Pectobacterium atrosepticum SCRI1043

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q6D3E1
• Locus tag: ECA_RS13760
• Protein ID: WP_011094338.1
• Coordinates: 3144548..3145018 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q6D3E0
• Locus tag: ECA_RS13765
• Protein ID: WP_011094339.1
• Coordinates: 3145015..3145458 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECA_RS13740	3139631..3140563	-	933	WP_011094334.1	tRNA dihydrouridine(16) synthase DusC	-
ECA_RS13745	3140808..3141479	+	672	WP_011094335.1	epoxyqueuosine reductase QueH	-
ECA_RS13750	3141526..3143121	-	1596	WP_011094336.1	ABC transporter substrate-binding protein	-
ECA_RS13755	3143262..3144491	+	1230	WP_011094337.1	peptidase T	-
ECA_RS13760	3144548..3145018	-	471	WP_011094338.1	RES family NAD+ phosphorylase	Toxin
ECA_RS13765	3145015..3145458	-	444	WP_011094339.1	DUF2384 domain-containing protein	Antitoxin
ECA_RS23435	3145527..3145631	+	105	Protein_2746	EamA family transporter	-
ECA_RS13770	3145672..3147123	-	1452	WP_011094340.1	ATP-dependent RNA helicase RhlE	-
ECA_RS13775	3147401..3148240	-	840	WP_011094341.1	ABC transporter ATP-binding protein	-
ECA_RS13780	3148253..3149284	-	1032	WP_011094342.1	iron ABC transporter permease	-
ECA_RS13785	3149277..3150266	-	990	WP_011094343.1	iron ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-145)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-144)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17623.06 Da
• Isoelectric Point: 4.4093

>T20865 WP_011094338.1 NC_004547:c3145018-3144548 [Pectobacterium atrosepticum SCRI1043]
MILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGTSMVYVSETASLAMLETLVHLHAAQILDYYTLMRIDVPEDQIQSADMD
ELPDNWAAEEAPPDLAVYGDAWIFTKGSIALRVPSALSPVEYNYLLNPEHPDFYGIIQMAEIIPFQFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T20865 NC_004547:c3145018-3144548 [Pectobacterium atrosepticum SCRI1043]
TTGATTCTCTATCGGCTTACAAAGACAAAGTATCTTTCTACAGCCTGGACGGGATACGGAGCAAAAGAGGCTGGAGGCCG
CTGGAACAGTGTTGGCACCTCGATGGTTTATGTATCAGAAACCGCATCGCTTGCCATGCTGGAGACGCTGGTACACTTGC
ATGCAGCGCAGATCCTGGATTATTACACGCTAATGCGTATCGATGTGCCTGAGGATCAGATTCAGAGTGCCGATATGGAC
GAATTGCCAGACAACTGGGCCGCTGAAGAAGCGCCGCCAGATCTGGCTGTATACGGTGATGCATGGATCTTTACCAAAGG
TTCGATAGCGTTGCGAGTCCCCAGCGCCCTATCGCCTGTGGAGTATAACTACCTGTTAAACCCTGAACACCCGGATTTCT
ACGGGATCATTCAGATGGCAGAGATTATACCGTTCCAGTTTGATAGCCGGCTAAAGCCTGATCGTAAATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.71 Da
• Isoelectric Point: 9.5903

>AT20865 WP_011094339.1 NC_004547:c3145458-3145015 [Pectobacterium atrosepticum SCRI1043]
MKTFSLSTTQTRPPRLWQVAGLNNSDGVALLGQINEGLEGKVANLIADWAKITQNDLRKMSGIPSTTFSRSVKARFSADQ
SERLVRIIRVIDRTVELFEGDKDEAQKWLNEPNRALGGKVPAELMVSETGAYEVMKLLTRLEHGVYS

Nucleotide

• Length: 444 bp

>AT20865 NC_004547:c3145458-3145015 [Pectobacterium atrosepticum SCRI1043]
ATGAAAACATTTAGCTTATCCACGACTCAGACTAGGCCGCCCCGTTTATGGCAGGTGGCCGGATTAAACAATTCTGATGG
TGTGGCGCTTCTGGGACAGATTAACGAAGGCCTTGAGGGCAAGGTTGCTAACCTTATCGCTGACTGGGCGAAGATAACGC
AAAACGATCTGCGTAAAATGTCCGGCATTCCGTCAACGACGTTTAGCCGCAGCGTCAAAGCGCGATTTAGCGCCGATCAA
AGTGAACGGCTGGTGCGTATCATCCGTGTCATCGATCGGACGGTAGAACTGTTCGAAGGTGATAAGGACGAGGCTCAAAA
ATGGTTGAATGAGCCAAACAGGGCGCTTGGCGGGAAAGTGCCAGCAGAACTGATGGTATCCGAAACCGGGGCTTATGAAG
TCATGAAATTGCTCACGCGCCTGGAACATGGTGTGTACTCTTGA

Structures

Toxin

• AlphaFold DB: Q6D3E1

Antitoxin

• AlphaFold DB: Q6D3E0