Detailed information of TA system TA020864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1923957..1924540
• Replicon: chromosome
• Accession: NC_004547
• Organism: Pectobacterium atrosepticum SCRI1043

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q6D6K4
• Locus tag: ECA_RS08285
• Protein ID: WP_011093255.1
• Coordinates: 1923957..1924289 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q6D6K3
• Locus tag: ECA_RS08290
• Protein ID: WP_011093256.1
• Coordinates: 1924289..1924540 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECA_RS22780	1919882..1920076	+	195	WP_011093247.1	hypothetical protein	-
ECA_RS08265	1920492..1920980	-	489	WP_011093248.1	hypothetical protein	-
ECA_RS23545	1921648..1921818	-	171	WP_164477417.1	hypothetical protein	-
ECA_RS22790	1922556..1922765	+	210	WP_080728693.1	hypothetical protein	-
ECA_RS08275	1922743..1922949	-	207	WP_011093251.1	hypothetical protein	-
ECA_RS22795	1923048..1923188	-	141	Protein_1633	integrase	-
ECA_RS08280	1923212..1923523	-	312	WP_039295601.1	hypothetical protein	-
ECA_RS23550	1923640..1923753	-	114	Protein_1635	integrase	-
ECA_RS08285	1923957..1924289	-	333	WP_011093255.1	endoribonuclease MazF	Toxin
ECA_RS08290	1924289..1924540	-	252	WP_011093256.1	PbsX family transcriptional regulator	Antitoxin
ECA_RS08295	1925154..1925486	-	333	WP_011093257.1	hypothetical protein	-
ECA_RS08300	1925682..1926959	-	1278	WP_011093258.1	integrase arm-type DNA-binding domain-containing protein	-
ECA_RS08310	1927325..1928122	-	798	WP_011093259.1	DgsA anti-repressor MtfA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-109)

Antitoxin

• Antitoxin PemI-like (3-82)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12162.97 Da
• Isoelectric Point: 7.0821

>T20864 WP_011093255.1 NC_004547:c1924289-1923957 [Pectobacterium atrosepticum SCRI1043]
MVSRFVPDSGDLIWLDFDPVVGHEQGGHRPAVVLSPFPYNNLTGMCLCVPCTTQRKGYNFEVELSGERESVALADQVTCV
DWRNRKATKKGKVTADELELIRAKAKALIG

Nucleotide

• Length: 333 bp

>T20864 NC_004547:c1924289-1923957 [Pectobacterium atrosepticum SCRI1043]
ATGGTTTCTCGTTTTGTTCCTGATTCCGGCGATTTGATCTGGTTAGATTTTGATCCGGTAGTGGGGCATGAACAGGGAGG
ACATCGCCCCGCTGTTGTTCTTAGCCCTTTTCCTTATAATAATTTGACCGGAATGTGCTTATGCGTCCCTTGTACGACCC
AGCGCAAAGGGTATAACTTCGAAGTCGAATTATCTGGAGAGCGTGAAAGCGTGGCTCTCGCCGATCAGGTTACTTGTGTA
GACTGGCGTAACCGTAAGGCAACAAAGAAAGGCAAAGTAACAGCCGATGAGTTAGAACTCATCAGAGCAAAAGCGAAGGC
TTTGATTGGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9452.94 Da
• Isoelectric Point: 4.7914

>AT20864 WP_011093256.1 NC_004547:c1924540-1924289 [Pectobacterium atrosepticum SCRI1043]
MIDVPVKKWGNSPAIRLSSSVMQAFDMTFNDSFDMEIRETEIALIPKKKPKYQFTLDELLEGMTPEHVHDKVDFGAPVGK
ELL

Nucleotide

• Length: 252 bp

>AT20864 NC_004547:c1924540-1924289 [Pectobacterium atrosepticum SCRI1043]
ATGATCGATGTCCCGGTTAAAAAATGGGGAAACTCACCTGCAATACGTCTTTCCTCATCTGTTATGCAAGCGTTTGATAT
GACGTTTAACGATAGCTTTGACATGGAGATCAGGGAAACAGAGATTGCTCTGATCCCCAAGAAAAAACCCAAATATCAGT
TTACGCTGGATGAGTTGCTCGAAGGGATGACGCCTGAACATGTCCATGACAAAGTTGATTTTGGCGCACCAGTCGGCAAG
GAGCTGCTTTAA

Structures

Toxin

• AlphaFold DB: Q6D6K4

Antitoxin

• AlphaFold DB: Q6D6K3