Detailed information of TA system TA208634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 598909..599438
• Replicon: chromosome
• Accession: NZ_CP077924
• Organism: Staphylococcus aureus strain 200

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU536_RS03080
• Protein ID: WP_000621175.1
• Coordinates: 599076..599438 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU536_RS03075
• Protein ID: WP_000948331.1
• Coordinates: 598909..599079 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU536_RS03045 (593945)	593945..594505	+	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-
KU536_RS03050 (594714)	594714..595193	+	480	WP_001287081.1	hypothetical protein	-
KU536_RS03055 (595186)	595186..596763	+	1578	WP_001294650.1	PH domain-containing protein	-
KU536_RS03060 (596756)	596756..597247	+	492	WP_001286980.1	PH domain-containing protein	-
KU536_RS03065 (597251)	597251..597610	+	360	WP_000581197.1	holo-ACP synthase	-
KU536_RS03070 (597676)	597676..598824	+	1149	WP_001281139.1	alanine racemase	-
KU536_RS03075 (598909)	598909..599079	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU536_RS03080 (599076)	599076..599438	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU536_RS03085 (599787)	599787..600788	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU536_RS03090 (600907)	600907..601233	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU536_RS03095 (601235)	601235..601714	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU536_RS03100 (601689)	601689..602459	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208634 WP_000621175.1 NZ_CP077924:599076-599438 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208634 NZ_CP103694:3633349-3633627 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208634 WP_000948331.1 NZ_CP077924:598909-599079 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208634 NZ_CP103694:3633086-3633346 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1