Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 579187..579701 Replicon chromosome
Accession NZ_CP077924
Organism Staphylococcus aureus strain 200

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU536_RS02990 Protein ID WP_000581792.1
Coordinates 579492..579701 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU536_RS02985 Protein ID WP_000138932.1
Coordinates 579187..579480 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU536_RS02965 (574950) 574950..576265 + 1316 Protein_583 ISL3 family transposase -
KU536_RS02970 (576243) 576243..576305 - 63 Protein_584 hypothetical protein -
KU536_RS02975 (576862) 576862..577509 - 648 WP_001187614.1 HD domain-containing protein -
KU536_RS02980 (577537) 577537..579021 - 1485 WP_000571550.1 cardiolipin synthase -
KU536_RS02985 (579187) 579187..579480 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU536_RS02990 (579492) 579492..579701 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU536_RS02995 (579829) 579829..579966 + 138 WP_000828354.1 Lmo0850 family protein -
KU536_RS03000 (580230) 580230..581432 - 1203 WP_020978293.1 FtsW/RodA/SpoVE family cell cycle protein -
KU536_RS03005 (581750) 581750..582820 + 1071 WP_000159641.1 D-alanine--D-alanine ligase -
KU536_RS03010 (582835) 582835..584190 + 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208633 WP_000581792.1 NZ_CP077924:579492-579701 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208633 NZ_CP103694:c3627288-3626890 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208633 WP_000138932.1 NZ_CP077924:579187-579480 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208633 NZ_CP103694:c3627584-3627291 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References