Detailed information of TA system TA020862

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 832337..832997
• Replicon: chromosome
• Accession: NC_004547
• Organism: Pectobacterium atrosepticum SCRI1043

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: Q6D959
• Locus tag: ECA_RS03775
• Protein ID: WP_011092367.1
• Coordinates: 832584..832997 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: Q6D960
• Locus tag: ECA_RS03770
• Protein ID: WP_011092366.1
• Coordinates: 832337..832603 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECA_RS03750	828144..829148	-	1005	WP_011092362.1	LacI family transcriptional regulator	-
ECA_RS03755	829202..829810	-	609	WP_011092363.1	HD domain-containing protein	-
ECA_RS03760	830229..830876	+	648	WP_011092364.1	hemolysin III family protein	-
ECA_RS03765	831100..832101	-	1002	WP_011092365.1	tRNA-modifying protein YgfZ	-
ECA_RS03770	832337..832603	+	267	WP_011092366.1	FAD assembly factor SdhE	Antitoxin
ECA_RS03775	832584..832997	+	414	WP_011092367.1	protein YgfX	Toxin
ECA_RS03780	833305..834240	+	936	WP_011092368.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
ECA_RS03785	834358..834681	-	324	WP_011092369.1	hypothetical protein	-
ECA_RS03790	834710..835228	-	519	WP_011092370.1	flavodoxin FldB	-
ECA_RS23350	835456..835632	+	177	WP_011092371.1	hypothetical protein	-
ECA_RS03795	835647..836093	-	447	WP_011092372.1	DUF1311 domain-containing protein	-
ECA_RS22390	836294..836902	-	609	WP_011092373.1	hypothetical protein	-
ECA_RS03805	837169..837669	-	501	WP_011092374.1	DUF4189 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16397.31 Da
• Isoelectric Point: 10.3509

>T20862 WP_011092367.1 NC_004547:832584-832997 [Pectobacterium atrosepticum SCRI1043]
VAQWQCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGVLLALQSINDKERQQLWLASDSMGDDEWRHLRQLLLQQKHWAR

Nucleotide

• Length: 414 bp

>T20862 NC_004547:832584-832997 [Pectobacterium atrosepticum SCRI1043]
GTGGCCCAGTGGCAATGTGACCTTCGTGTTTCCTGGCGCATGCAACTGCTGTCATTGGTTGTGCATGGCCTACTCGTATT
GCTCATTCTGCTGGCACCGTGGCCGGACGGTTACGCATGGCTATGGCTATGCTTAGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAAAGGAATATCAAATCCCGACAGGGGGAAATCGTTTTGCTTAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTCAAACGACCGTGGCTTTTGAAAAATGGCGTGTTGTTGGCATTGCAATCTATCAACGATAAAGAAAG
ACAACAGCTGTGGCTGGCATCGGACAGTATGGGCGATGATGAGTGGCGCCATCTGCGTCAGCTTCTTTTGCAGCAGAAAC
ACTGGGCGCGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10462.94 Da
• Isoelectric Point: 6.2223

>AT20862 WP_011092366.1 NC_004547:832337-832603 [Pectobacterium atrosepticum SCRI1043]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDSDKRAFVRLLQSDDPDLFNWLMNHGEPKDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT20862 NC_004547:832337-832603 [Pectobacterium atrosepticum SCRI1043]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGTGAACTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACAGTGATAAGCGCGCGTTTGTACGCCTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGAAGGACGGAGAGTTGAAACGTATGATTTCCCTTATTCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: Q6D959

Antitoxin

• AlphaFold DB: Q6D960