Detailed information of TA system TA208617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 546252..546781
• Replicon: chromosome
• Accession: NZ_CP077923
• Organism: Staphylococcus aureus strain 201

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU534_RS02810
• Protein ID: WP_000621175.1
• Coordinates: 546419..546781 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU534_RS02805
• Protein ID: WP_000948331.1
• Coordinates: 546252..546422 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU534_RS02775 (541288)	541288..541848	+	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-
KU534_RS02780 (542057)	542057..542536	+	480	WP_001287081.1	hypothetical protein	-
KU534_RS02785 (542529)	542529..544106	+	1578	WP_001294650.1	PH domain-containing protein	-
KU534_RS02790 (544099)	544099..544590	+	492	WP_001286980.1	PH domain-containing protein	-
KU534_RS02795 (544594)	544594..544953	+	360	WP_000581197.1	holo-ACP synthase	-
KU534_RS02800 (545019)	545019..546167	+	1149	WP_001281139.1	alanine racemase	-
KU534_RS02805 (546252)	546252..546422	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU534_RS02810 (546419)	546419..546781	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU534_RS02815 (547130)	547130..548131	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU534_RS02820 (548250)	548250..548576	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU534_RS02825 (548578)	548578..549057	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU534_RS02830 (549032)	549032..549802	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208617 WP_000621175.1 NZ_CP077923:546419-546781 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208617 NZ_CP103687:c5286918-5286535 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208617 WP_000948331.1 NZ_CP077923:546252-546422 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208617 NZ_CP103687:c5287160-5286918 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1