Detailed information of TA system TA020860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 466567..467094
• Replicon: chromosome
• Accession: NC_004547
• Organism: Pectobacterium atrosepticum SCRI1043

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q6DA47
• Locus tag: ECA_RS02100
• Protein ID: WP_011092038.1
• Coordinates: 466819..467094 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ECA_RS02095
• Protein ID: WP_043877996.1
• Coordinates: 466567..466815 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECA_RS02075	461737..463248	-	1512	WP_011092033.1	leucyl aminopeptidase	-
ECA_RS02080	463531..464646	+	1116	WP_011092034.1	LPS export ABC transporter permease LptF	-
ECA_RS02085	464646..465722	+	1077	WP_011092035.1	LPS export ABC transporter permease LptG	-
ECA_RS22505	466210..466374	+	165	Protein_399	DUF4102 domain-containing protein	-
ECA_RS22510	466362..466457	+	96	Protein_400	DUF4942 domain-containing protein	-
ECA_RS02095	466567..466815	+	249	WP_043877996.1	ribbon-helix-helix domain-containing protein	Antitoxin
ECA_RS02100	466819..467094	+	276	WP_011092038.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECA_RS02105	467142..467654	+	513	WP_011092039.1	AAA family ATPase	-
ECA_RS23605	468116..469358	+	1243	Protein_404	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	466210..475612	9402

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11003.24 Da
• Isoelectric Point: 5.1487

>T20860 WP_011092038.1 NC_004547:466819-467094 [Pectobacterium atrosepticum SCRI1043]
MEIRWTQKAQDDLERIYGFASQYSRQHADEVLDRLIIGTTGLADHPRIGVSQARYEPREVRKILLDDYEFHYEIQHDTIY
IVDLWHTREDR

Nucleotide

• Length: 276 bp

>T20860 NC_004547:466819-467094 [Pectobacterium atrosepticum SCRI1043]
ATGGAAATACGTTGGACGCAAAAGGCACAGGATGACCTAGAACGTATTTATGGGTTTGCCAGCCAGTACAGTCGGCAACA
TGCTGATGAGGTGTTAGATCGCTTGATCATCGGAACCACGGGGCTTGCTGACCACCCGAGAATCGGCGTATCTCAGGCAA
GATATGAACCACGAGAGGTAAGAAAGATTTTGCTTGATGATTATGAATTTCACTATGAAATTCAGCATGATACGATTTAT
ATCGTCGATCTTTGGCATACACGAGAAGATCGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8979.33 Da
• Isoelectric Point: 7.2045

>AT20860 WP_043877996.1 NC_004547:466567-466815 [Pectobacterium atrosepticum SCRI1043]
MGQPAKQTISAQIPAELAAAVESLAIELDRSKSWVIKEALTAMIEERERRHQQVLKGLADVDAGRVVSHADVIDFANKLK
KS

Nucleotide

• Length: 249 bp

>AT20860 NC_004547:466567-466815 [Pectobacterium atrosepticum SCRI1043]
ATGGGACAACCCGCGAAACAGACGATCAGTGCTCAGATACCCGCTGAGCTTGCTGCTGCGGTTGAAAGCCTGGCTATTGA
ATTGGATAGATCCAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCAATGATTGAAGAGAGAGAACGGCGGCATCAGCAGG
TTTTGAAAGGCCTTGCTGATGTTGATGCGGGAAGGGTGGTAAGCCATGCTGATGTGATCGACTTCGCCAACAAACTGAAA
AAGTCATAA

Structures

Toxin

• AlphaFold DB: Q6DA47