Detailed information of TA system TA208595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2597795..2598324
• Replicon: chromosome
• Accession: NZ_CP077921
• Organism: Staphylococcus aureus strain 276

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU530_RS12755
• Protein ID: WP_000621175.1
• Coordinates: 2597962..2598324 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU530_RS12750
• Protein ID: WP_000948331.1
• Coordinates: 2597795..2597965 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU530_RS12720 (KU530_12670)	2592831..2593391	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
KU530_RS12725 (KU530_12675)	2593600..2594079	+	480	WP_001287079.1	hypothetical protein	-
KU530_RS12730 (KU530_12680)	2594072..2595655	+	1584	WP_001294620.1	PH domain-containing protein	-
KU530_RS12735 (KU530_12685)	2595642..2596133	+	492	WP_001205912.1	PH domain-containing protein	-
KU530_RS12740 (KU530_12690)	2596137..2596496	+	360	WP_000581202.1	holo-ACP synthase	-
KU530_RS12745 (KU530_12695)	2596562..2597710	+	1149	WP_000130547.1	alanine racemase	-
KU530_RS12750 (KU530_12700)	2597795..2597965	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU530_RS12755 (KU530_12705)	2597962..2598324	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU530_RS12760 (KU530_12710)	2598673..2599674	+	1002	WP_225806583.1	PP2C family protein-serine/threonine phosphatase	-
KU530_RS12765 (KU530_12715)	2599793..2600119	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU530_RS12770 (KU530_12720)	2600121..2600600	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU530_RS12775 (KU530_12725)	2600575..2601345	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208595 WP_000621175.1 NZ_CP077921:2597962-2598324 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208595 NZ_CP103683:c2319727-2319242 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATGATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208595 WP_000948331.1 NZ_CP077921:2597795-2597965 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208595 NZ_CP103683:c2319981-2319715 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTTAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1