Detailed information of TA system TA208582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1899906..1900435
• Replicon: chromosome
• Accession: NZ_CP077920
• Organism: Staphylococcus aureus strain 277

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU525_RS09360
• Protein ID: WP_000621175.1
• Coordinates: 1900073..1900435 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU525_RS09355
• Protein ID: WP_000948331.1
• Coordinates: 1899906..1900076 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU525_RS09325 (1894942)	1894942..1895502	+	561	WP_001092416.1	K(+)-transporting ATPase subunit C	-
KU525_RS09330 (1895711)	1895711..1896190	+	480	WP_001287090.1	hypothetical protein	-
KU525_RS09335 (1896183)	1896183..1897766	+	1584	WP_001294626.1	PH domain-containing protein	-
KU525_RS09340 (1897753)	1897753..1898244	+	492	WP_001205910.1	PH domain-containing protein	-
KU525_RS09345 (1898248)	1898248..1898607	+	360	WP_000581200.1	holo-ACP synthase	-
KU525_RS09350 (1898673)	1898673..1899821	+	1149	WP_001281151.1	alanine racemase	-
KU525_RS09355 (1899906)	1899906..1900076	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU525_RS09360 (1900073)	1900073..1900435	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU525_RS09365 (1900784)	1900784..1901785	+	1002	WP_061839378.1	PP2C family protein-serine/threonine phosphatase	-
KU525_RS09370 (1901904)	1901904..1902230	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU525_RS09375 (1902232)	1902232..1902711	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU525_RS09380 (1902686)	1902686..1903456	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208582 WP_000621175.1 NZ_CP077920:1900073-1900435 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208582 NZ_CP103679:c2866169-2865816 [Enterobacter hormaechei]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTTTTGCGGGAGAGGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGGTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208582 WP_000948331.1 NZ_CP077920:1899906-1900076 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208582 NZ_CP103679:c2865811-2865509 [Enterobacter hormaechei]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGACGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTACCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1