Detailed information of TA system TA208569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 352515..352992
• Replicon: chromosome
• Accession: NZ_CP077920
• Organism: Staphylococcus aureus strain 277

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: KU525_RS01770
• Protein ID: WP_000052076.1
• Coordinates: 352804..352992 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: KU525_RS01765
• Protein ID: WP_001059690.1
• Coordinates: 352515..352775 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU525_RS01730 (347910)	347910..348626	-	717	WP_000150021.1	type I 3-dehydroquinate dehydratase	-
KU525_RS01735 (348774)	348774..349196	+	423	WP_000974447.1	organic hydroperoxide resistance protein	-
KU525_RS01740 (349595)	349595..350089	+	495	WP_064626748.1	GNAT family N-acetyltransferase	-
KU525_RS01745 (350243)	350243..350860	+	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
KU525_RS01750 (350933)	350933..351514	-	582	WP_001140690.1	histidine phosphatase family protein	-
KU525_RS01755 (351739)	351739..351975	+	237	WP_000368687.1	hypothetical protein	-
KU525_RS01760 (351972)	351972..352175	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
KU525_RS01765 (352515)	352515..352775	+	261	WP_001059690.1	hypothetical protein	Antitoxin
KU525_RS01770 (352804)	352804..352992	+	189	WP_000052076.1	hypothetical protein	Toxin
KU525_RS01775 (353180)	353180..353749	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
KU525_RS01780 (353837)	353837..354121	+	285	WP_001004395.1	hypothetical protein	-
KU525_RS01785 (354183)	354183..354401	+	219	WP_000005889.1	hypothetical protein	-
KU525_RS01790 (354896)	354896..355096	-	201	WP_001059082.1	cold-shock protein	-
KU525_RS01795 (355453)	355453..356139	-	687	WP_001641381.1	thermonuclease family protein	-
KU525_RS01800 (356480)	356480..356989	-	510	WP_000824480.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T208569 WP_000052076.1 NZ_CP077920:352804-352992 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T208569 NZ_CP103676:3989872-3990090 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT208569 WP_001059690.1 NZ_CP077920:352515-352775 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT208569 NZ_CP103676:3989471-3989845 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56