Detailed information of TA system TA208567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2522312..2522841
• Replicon: chromosome
• Accession: NZ_CP077919
• Organism: Staphylococcus aureus strain 278

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU514_RS12565
• Protein ID: WP_000621175.1
• Coordinates: 2522479..2522841 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU514_RS12560
• Protein ID: WP_000948331.1
• Coordinates: 2522312..2522482 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU514_RS12530 (KU514_12455)	2517348..2517908	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
KU514_RS12535 (KU514_12460)	2518117..2518596	+	480	WP_001287079.1	hypothetical protein	-
KU514_RS12540 (KU514_12465)	2518589..2520172	+	1584	WP_001294620.1	PH domain-containing protein	-
KU514_RS12545 (KU514_12470)	2520159..2520650	+	492	WP_001205912.1	PH domain-containing protein	-
KU514_RS12550 (KU514_12475)	2520654..2521013	+	360	WP_000581202.1	holo-ACP synthase	-
KU514_RS12555 (KU514_12480)	2521079..2522227	+	1149	WP_000130547.1	alanine racemase	-
KU514_RS12560 (KU514_12485)	2522312..2522482	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU514_RS12565 (KU514_12490)	2522479..2522841	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU514_RS12570 (KU514_12495)	2523190..2524191	+	1002	WP_225806583.1	PP2C family protein-serine/threonine phosphatase	-
KU514_RS12575 (KU514_12500)	2524310..2524636	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU514_RS12580 (KU514_12505)	2524638..2525117	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU514_RS12585 (KU514_12510)	2525092..2525862	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208567 WP_000621175.1 NZ_CP077919:2522479-2522841 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208567 NZ_CP103676:2394779-2395096 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208567 WP_000948331.1 NZ_CP077919:2522312-2522482 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208567 NZ_CP103676:2395172-2395468 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1