Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2502569..2503083 Replicon chromosome
Accession NZ_CP077919
Organism Staphylococcus aureus strain 278

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU514_RS12475 Protein ID WP_000581792.1
Coordinates 2502874..2503083 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU514_RS12470 Protein ID WP_000138932.1
Coordinates 2502569..2502862 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU514_RS12450 (KU514_12375) 2497918..2498559 + 642 WP_000483153.1 thiamine phosphate synthase -
KU514_RS12455 (KU514_12380) 2498645..2499517 + 873 WP_000725802.1 membrane protein insertase YidC -
KU514_RS12460 (KU514_12385) 2500244..2500891 - 648 WP_001187634.1 HD domain-containing protein -
KU514_RS12465 (KU514_12390) 2500919..2502403 - 1485 WP_000571549.1 cardiolipin synthase -
KU514_RS12470 (KU514_12395) 2502569..2502862 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU514_RS12475 (KU514_12400) 2502874..2503083 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU514_RS12480 (KU514_12405) 2503211..2503348 + 138 WP_000828354.1 Lmo0850 family protein -
KU514_RS12485 (KU514_12410) 2503612..2504814 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU514_RS12490 (KU514_12415) 2505132..2506202 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
KU514_RS12495 (KU514_12420) 2506217..2507575 + 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208566 WP_000581792.1 NZ_CP077919:2502874-2503083 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208566 NZ_CP103676:1925888-1925990 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208566 WP_000138932.1 NZ_CP077919:2502569-2502862 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208566 NZ_CP103676:c1925997-1925852 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References