Detailed information of TA system TA208545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 492481..493010
• Replicon: chromosome
• Accession: NZ_CP077917
• Organism: Staphylococcus aureus strain 280

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU509_RS02575
• Protein ID: WP_000621175.1
• Coordinates: 492481..492843 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU509_RS02580
• Protein ID: WP_000948331.1
• Coordinates: 492840..493010 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU509_RS02555 (489460)	489460..490230	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
KU509_RS02560 (490205)	490205..490684	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU509_RS02565 (490686)	490686..491012	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU509_RS02570 (491131)	491131..492132	-	1002	WP_061839378.1	PP2C family protein-serine/threonine phosphatase	-
KU509_RS02575 (492481)	492481..492843	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU509_RS02580 (492840)	492840..493010	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU509_RS02585 (493095)	493095..494243	-	1149	WP_001281151.1	alanine racemase	-
KU509_RS02590 (494309)	494309..494668	-	360	WP_000581200.1	holo-ACP synthase	-
KU509_RS02595 (494672)	494672..495163	-	492	WP_001205910.1	PH domain-containing protein	-
KU509_RS02600 (495150)	495150..496733	-	1584	WP_001294626.1	PH domain-containing protein	-
KU509_RS02605 (496726)	496726..497205	-	480	WP_001287090.1	hypothetical protein	-
KU509_RS02610 (497414)	497414..497974	-	561	WP_001092416.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208545 WP_000621175.1 NZ_CP077917:c492843-492481 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208545 NZ_CP103673:c2794908-2794805 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208545 WP_000948331.1 NZ_CP077917:c493010-492840 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208545 NZ_CP103673:2794767-2795038 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1