Detailed information of TA system TA208529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2521931..2522460
• Replicon: chromosome
• Accession: NZ_CP077915
• Organism: Staphylococcus aureus strain 282

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU535_RS12240
• Protein ID: WP_000621175.1
• Coordinates: 2522098..2522460 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU535_RS12235
• Protein ID: WP_000948331.1
• Coordinates: 2521931..2522101 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU535_RS12205 (KU535_12150)	2516967..2517527	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
KU535_RS12210 (KU535_12155)	2517736..2518215	+	480	WP_001287079.1	hypothetical protein	-
KU535_RS12215 (KU535_12160)	2518208..2519791	+	1584	WP_001294620.1	PH domain-containing protein	-
KU535_RS12220 (KU535_12165)	2519778..2520269	+	492	WP_001205912.1	PH domain-containing protein	-
KU535_RS12225 (KU535_12170)	2520273..2520632	+	360	WP_000581202.1	holo-ACP synthase	-
KU535_RS12230 (KU535_12175)	2520698..2521846	+	1149	WP_000130547.1	alanine racemase	-
KU535_RS12235 (KU535_12180)	2521931..2522101	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU535_RS12240 (KU535_12185)	2522098..2522460	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU535_RS12245 (KU535_12190)	2522809..2523810	+	1002	WP_225806583.1	PP2C family protein-serine/threonine phosphatase	-
KU535_RS12250 (KU535_12195)	2523929..2524255	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU535_RS12255 (KU535_12200)	2524257..2524736	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU535_RS12260 (KU535_12205)	2524711..2525481	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208529 WP_000621175.1 NZ_CP077915:2522098-2522460 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208529 NZ_CP103672:c5177217-5176906 [Escherichia coli O25b:H4-ST131]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208529 WP_000948331.1 NZ_CP077915:2521931-2522101 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208529 NZ_CP103672:c5176905-5176615 [Escherichia coli O25b:H4-ST131]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1