208528

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2502188..2502702 Replicon chromosome
Accession NZ_CP077915
Organism Staphylococcus aureus strain 282

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU535_RS12150 Protein ID WP_000581792.1
Coordinates 2502493..2502702 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU535_RS12145 Protein ID WP_000138932.1
Coordinates 2502188..2502481 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU535_RS12125 (KU535_12070) 2497537..2498178 + 642 WP_000483153.1 thiamine phosphate synthase -
KU535_RS12130 (KU535_12075) 2498264..2499136 + 873 WP_000725802.1 membrane protein insertase YidC -
KU535_RS12135 (KU535_12080) 2499863..2500510 - 648 WP_001187634.1 HD domain-containing protein -
KU535_RS12140 (KU535_12085) 2500538..2502022 - 1485 WP_000571549.1 cardiolipin synthase -
KU535_RS12145 (KU535_12090) 2502188..2502481 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU535_RS12150 (KU535_12095) 2502493..2502702 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU535_RS12155 (KU535_12100) 2502830..2502967 + 138 WP_000828354.1 Lmo0850 family protein -
KU535_RS12160 (KU535_12105) 2503231..2504433 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU535_RS12165 (KU535_12110) 2504751..2505821 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
KU535_RS12170 (KU535_12115) 2505836..2507194 + 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208528 WP_000581792.1 NZ_CP077915:2502493-2502702 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208528 NZ_CP103672:c4860889-4860716 [Escherichia coli O25b:H4-ST131]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208528 WP_000138932.1 NZ_CP077915:2502188-2502481 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208528 NZ_CP103672:c4861213-4860917 [Escherichia coli O25b:H4-ST131]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References