Detailed information of TA system TA208511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2248343..2248872
• Replicon: chromosome
• Accession: NZ_CP077911
• Organism: Staphylococcus aureus strain 318

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU501_RS11285
• Protein ID: WP_225798545.1
• Coordinates: 2248343..2248705 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU501_RS11290
• Protein ID: WP_000948331.1
• Coordinates: 2248702..2248872 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU501_RS11265 (2245322)	2245322..2246092	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU501_RS11270 (2246067)	2246067..2246546	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU501_RS11275 (2246548)	2246548..2246874	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU501_RS11280 (2246993)	2246993..2247994	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU501_RS11285 (2248343)	2248343..2248705	-	363	WP_225798545.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU501_RS11290 (2248702)	2248702..2248872	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU501_RS11295 (2248957)	2248957..2250105	-	1149	WP_001281140.1	alanine racemase	-
KU501_RS11300 (2250171)	2250171..2250530	-	360	WP_000581197.1	holo-ACP synthase	-
KU501_RS11305 (2250534)	2250534..2251025	-	492	WP_001286801.1	PH domain-containing protein	-
KU501_RS11310 (2251018)	2251018..2252595	-	1578	WP_001294646.1	PH domain-containing protein	-
KU501_RS11315 (2252588)	2252588..2253067	-	480	WP_001287082.1	hypothetical protein	-
KU501_RS11320 (2253276)	2253276..2253836	-	561	WP_001092414.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13468.76 Da
• Isoelectric Point: 10.2892

>T208511 WP_225798545.1 NZ_CP077911:c2248705-2248343 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDNKMKEVDNALMISLGLNAVVHQKN

Nucleotide

• Length: 363 bp

>T208511 NZ_CP103672:641944-642258 [Escherichia coli O25b:H4-ST131]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208511 WP_000948331.1 NZ_CP077911:c2248872-2248702 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208511 NZ_CP103672:642255-642671 [Escherichia coli O25b:H4-ST131]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1