Detailed information of TA system TA208499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2701164..2701693
• Replicon: chromosome
• Accession: NZ_CP077908
• Organism: Staphylococcus aureus strain 322

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: KU527_RS13275
• Protein ID: WP_001103939.1
• Coordinates: 2701376..2701693 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: KU527_RS13270
• Protein ID: WP_001058494.1
• Coordinates: 2701164..2701373 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU527_RS13235 (2697494)	2697494..2697958	+	465	WP_001085185.1	SsrA-binding protein SmpB	-
KU527_RS13245 (2698521)	2698521..2699627	-	1107	WP_000149511.1	tyrosine-type recombinase/integrase	-
KU527_RS13250 (2699617)	2699617..2700420	-	804	WP_000358990.1	helix-turn-helix transcriptional regulator	-
KU527_RS13255 (2700556)	2700556..2700759	+	204	WP_001045296.1	transcriptional regulator	-
KU527_RS13260 (2700795)	2700795..2701013	+	219	WP_000163544.1	helix-turn-helix transcriptional regulator	-
KU527_RS13265 (2701025)	2701025..2701171	+	147	WP_000784885.1	hypothetical protein	-
KU527_RS13270 (2701164)	2701164..2701373	+	210	WP_001058494.1	hypothetical protein	Antitoxin
KU527_RS13275 (2701376)	2701376..2701693	+	318	WP_001103939.1	DUF1474 family protein	Toxin
KU527_RS13280 (2701757)	2701757..2702626	+	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
KU527_RS13285 (2702643)	2702643..2704100	+	1458	WP_000390453.1	virulence-associated E family protein	-
KU527_RS13290 (2704401)	2704401..2704763	+	363	WP_001039170.1	hypothetical protein	-
KU527_RS13295 (2704765)	2704765..2705049	+	285	WP_000998185.1	hypothetical protein	-
KU527_RS13300 (2705046)	2705046..2705687	+	642	WP_001019808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T208499 WP_001103939.1 NZ_CP077908:2701376-2701693 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T208499 NZ_CP103657:c4665460-4665074 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCTGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT208499 WP_001058494.1 NZ_CP077908:2701164-2701373 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT208499 NZ_CP103657:c4665999-4665490 [Escherichia coli]
ATGGCATATCTGAAATTGATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGA
AGATAGTCTTTACTCTATGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGCATTG
GTGACTACTGGTACAACTTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGAC
GAGGTCTTTGATGAGTTGAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATT
TGATCTCGCAAACATGACGCATTTAGAAAGCGTTTGTCCAGAGTGGCATAATCCTGGCAATTCTCGTAAGCCTATAGACC
TGAAAGAAATGCTGATCAGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAA
CTTAAGGAATTTTCTTTGCAATTATCATGA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IY59