Detailed information of TA system TA208495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1219449..1219978
• Replicon: chromosome
• Accession: NZ_CP077908
• Organism: Staphylococcus aureus strain 322

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU527_RS06155
• Protein ID: WP_000621175.1
• Coordinates: 1219449..1219811 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU527_RS06160
• Protein ID: WP_000948331.1
• Coordinates: 1219808..1219978 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU527_RS06135 (1216428)	1216428..1217198	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU527_RS06140 (1217173)	1217173..1217652	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU527_RS06145 (1217654)	1217654..1217980	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU527_RS06150 (1218099)	1218099..1219100	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU527_RS06155 (1219449)	1219449..1219811	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU527_RS06160 (1219808)	1219808..1219978	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU527_RS06165 (1220063)	1220063..1221211	-	1149	WP_001281140.1	alanine racemase	-
KU527_RS06170 (1221277)	1221277..1221636	-	360	WP_000581199.1	holo-ACP synthase	-
KU527_RS06175 (1221640)	1221640..1222131	-	492	WP_001205907.1	PH domain-containing protein	-
KU527_RS06180 (1222118)	1222118..1223701	-	1584	WP_001294637.1	PH domain-containing protein	-
KU527_RS06185 (1223694)	1223694..1224173	-	480	WP_001287077.1	hypothetical protein	-
KU527_RS06190 (1224381)	1224381..1224941	-	561	WP_225806291.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208495 WP_000621175.1 NZ_CP077908:c1219811-1219449 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208495 NZ_CP103657:c4218940-4218626 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208495 WP_000948331.1 NZ_CP077908:c1219978-1219808 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208495 NZ_CP103657:c4219176-4218943 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1