Detailed information of TA system TA208492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1026007..1026783
• Replicon: chromosome
• Accession: NZ_CP077908
• Organism: Staphylococcus aureus strain 322

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: KU527_RS04940
• Protein ID: WP_000031109.1
• Coordinates: 1026007..1026159 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: KU527_RS04945
• Protein ID: WP_001251224.1
• Coordinates: 1026184..1026783 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU527_RS04925 (1021813)	1021813..1022634	+	822	WP_000669377.1	RluA family pseudouridine synthase	-
KU527_RS04930 (1023091)	1023091..1024476	-	1386	WP_000116239.1	class II fumarate hydratase	-
KU527_RS04935 (1024672)	1024672..1025067	-	396	WP_000901019.1	hypothetical protein	-
KU527_RS04940 (1026007)	1026007..1026159	-	153	WP_000031109.1	SAS053 family protein	Toxin
KU527_RS04945 (1026184)	1026184..1026783	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
KU527_RS04950 (1026942)	1026942..1027412	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
KU527_RS04955 (1027417)	1027417..1028544	-	1128	WP_000379990.1	tRNA epoxyqueuosine(34) reductase QueG	-
KU527_RS04960 (1028695)	1028695..1029417	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
KU527_RS04965 (1029410)	1029410..1030867	-	1458	WP_000649916.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5950.31 Da
• Isoelectric Point: 3.9075

>T208492 WP_000031109.1 NZ_CP077908:c1026159-1026007 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEESQ

Nucleotide

• Length: 153 bp

>T208492 NZ_CP103657:c3710974-3710672 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT208492 WP_001251224.1 NZ_CP077908:c1026783-1026184 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT208492 NZ_CP103657:c3710636-3710295 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: W8U4V4