Detailed information of TA system TA208474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2046354..2046883
• Replicon: chromosome
• Accession: NZ_CP077898
• Organism: Staphylococcus aureus strain 327

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU503_RS10295
• Protein ID: WP_000621175.1
• Coordinates: 2046354..2046716 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU503_RS10300
• Protein ID: WP_000948331.1
• Coordinates: 2046713..2046883 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU503_RS10275 (2043333)	2043333..2044103	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU503_RS10280 (2044078)	2044078..2044557	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU503_RS10285 (2044559)	2044559..2044885	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU503_RS10290 (2045004)	2045004..2046005	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU503_RS10295 (2046354)	2046354..2046716	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU503_RS10300 (2046713)	2046713..2046883	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU503_RS10305 (2046968)	2046968..2048116	-	1149	WP_001281140.1	alanine racemase	-
KU503_RS10310 (2048182)	2048182..2048541	-	360	WP_000581199.1	holo-ACP synthase	-
KU503_RS10315 (2048545)	2048545..2049036	-	492	WP_001205907.1	PH domain-containing protein	-
KU503_RS10320 (2049023)	2049023..2050606	-	1584	WP_001294637.1	PH domain-containing protein	-
KU503_RS10325 (2050599)	2050599..2051078	-	480	WP_001287077.1	hypothetical protein	-
KU503_RS10330 (2051286)	2051286..2051846	-	561	WP_225806291.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208474 WP_000621175.1 NZ_CP077898:c2046716-2046354 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208474 NZ_CP103654:c5280032-5279649 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208474 WP_000948331.1 NZ_CP077898:c2046883-2046713 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208474 NZ_CP103654:c5280274-5280032 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1