Detailed information of TA system TA208469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 763834..764363
• Replicon: chromosome
• Accession: NZ_CP077898
• Organism: Staphylococcus aureus strain 327

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: KU503_RS03655
• Protein ID: WP_001103939.1
• Coordinates: 764046..764363 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: KU503_RS03650
• Protein ID: WP_001058494.1
• Coordinates: 763834..764043 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU503_RS03615 (760164)	760164..760628	+	465	WP_001085185.1	SsrA-binding protein SmpB	-
KU503_RS03625 (761191)	761191..762297	-	1107	WP_000149511.1	tyrosine-type recombinase/integrase	-
KU503_RS03630 (762287)	762287..763090	-	804	WP_000358990.1	helix-turn-helix transcriptional regulator	-
KU503_RS03635 (763226)	763226..763429	+	204	WP_001045296.1	transcriptional regulator	-
KU503_RS03640 (763465)	763465..763683	+	219	WP_000163544.1	helix-turn-helix transcriptional regulator	-
KU503_RS03645 (763695)	763695..763841	+	147	WP_000784885.1	hypothetical protein	-
KU503_RS03650 (763834)	763834..764043	+	210	WP_001058494.1	hypothetical protein	Antitoxin
KU503_RS03655 (764046)	764046..764363	+	318	WP_001103939.1	DUF1474 family protein	Toxin
KU503_RS03660 (764427)	764427..765296	+	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
KU503_RS03665 (765313)	765313..766770	+	1458	WP_000390453.1	virulence-associated E family protein	-
KU503_RS03670 (767071)	767071..767433	+	363	WP_001039170.1	hypothetical protein	-
KU503_RS03675 (767435)	767435..767719	+	285	WP_000998185.1	hypothetical protein	-
KU503_RS03680 (767716)	767716..768357	+	642	WP_001019808.1	hypothetical protein	-
KU503_RS13780 (768604)	768604..768738	+	135	WP_256094151.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T208469 WP_001103939.1 NZ_CP077898:764046-764363 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T208469 NZ_CP103654:4027538-4027756 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT208469 WP_001058494.1 NZ_CP077898:763834-764043 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT208469 NZ_CP103654:4027137-4027511 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IY59