Detailed information of TA system TA208463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1860324..1860853
• Replicon: chromosome
• Accession: NZ_CP077897
• Organism: Staphylococcus aureus strain 328

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU544_RS09210
• Protein ID: WP_000621175.1
• Coordinates: 1860491..1860853 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU544_RS09205
• Protein ID: WP_000948331.1
• Coordinates: 1860324..1860494 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU544_RS09175 (1855361)	1855361..1855921	+	561	WP_225806291.1	K(+)-transporting ATPase subunit C	-
KU544_RS09180 (1856129)	1856129..1856608	+	480	WP_001287077.1	hypothetical protein	-
KU544_RS09185 (1856601)	1856601..1858184	+	1584	WP_001294637.1	PH domain-containing protein	-
KU544_RS09190 (1858171)	1858171..1858662	+	492	WP_001205907.1	PH domain-containing protein	-
KU544_RS09195 (1858666)	1858666..1859025	+	360	WP_000581199.1	holo-ACP synthase	-
KU544_RS09200 (1859091)	1859091..1860239	+	1149	WP_001281140.1	alanine racemase	-
KU544_RS09205 (1860324)	1860324..1860494	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU544_RS09210 (1860491)	1860491..1860853	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU544_RS09215 (1861202)	1861202..1862203	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU544_RS09220 (1862322)	1862322..1862648	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU544_RS09225 (1862650)	1862650..1863129	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU544_RS09230 (1863104)	1863104..1863874	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208463 WP_000621175.1 NZ_CP077897:1860491-1860853 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208463 NZ_CP103654:325880-326248 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208463 WP_000948331.1 NZ_CP077897:1860324-1860494 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208463 NZ_CP103654:325662-325883 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1