Detailed information of TA system TA208457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 2455073..2455550
• Replicon: chromosome
• Accession: NZ_CP077894
• Organism: Staphylococcus aureus strain 329

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: KU540_RS12275
• Protein ID: WP_000052076.1
• Coordinates: 2455362..2455550 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: KU540_RS12270
• Protein ID: WP_001059690.1
• Coordinates: 2455073..2455333 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU540_RS12235 (2450466)	2450466..2451182	-	717	WP_000150034.1	type I 3-dehydroquinate dehydratase	-
KU540_RS12240 (2451330)	2451330..2451752	+	423	WP_000974451.1	organic hydroperoxide resistance protein	-
KU540_RS12245 (2452151)	2452151..2452645	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
KU540_RS12250 (2452801)	2452801..2453418	+	618	WP_000255550.1	LysE/ArgO family amino acid transporter	-
KU540_RS12255 (2453491)	2453491..2454072	-	582	WP_001140696.1	histidine phosphatase family protein	-
KU540_RS12260 (2454296)	2454296..2454532	+	237	WP_000368687.1	hypothetical protein	-
KU540_RS12265 (2454529)	2454529..2454732	-	204	WP_000145160.1	sterile alpha motif-like domain-containing protein	-
KU540_RS12270 (2455073)	2455073..2455333	+	261	WP_001059690.1	hypothetical protein	Antitoxin
KU540_RS12275 (2455362)	2455362..2455550	+	189	WP_000052076.1	hypothetical protein	Toxin
KU540_RS12280 (2455738)	2455738..2456307	+	570	WP_001106662.1	lipoprotein N-acylation protein LnsA	-
KU540_RS12285 (2456395)	2456395..2456679	+	285	WP_001004401.1	hypothetical protein	-
KU540_RS12290 (2456741)	2456741..2456959	+	219	WP_000005884.1	hypothetical protein	-
KU540_RS12295 (2457484)	2457484..2457684	-	201	WP_001059082.1	cold-shock protein	-
KU540_RS12300 (2458047)	2458047..2458715	-	669	WP_000141442.1	thermonuclease family protein	-
KU540_RS12305 (2459057)	2459057..2459527	-	471	WP_000824466.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp / sell / sec / tsst-1 / sell / sec / tsst-1	2451330..2499223	47893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T208457 WP_000052076.1 NZ_CP077894:2455362-2455550 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T208457 NZ_CP103645:c4454890-4454717 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT208457 WP_001059690.1 NZ_CP077894:2455073-2455333 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT208457 NZ_CP103645:c4455214-4454918 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56