Detailed information of TA system TA208436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 515981..516510
• Replicon: chromosome
• Accession: NZ_CP077893
• Organism: Staphylococcus aureus strain 332

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU542_RS02645
• Protein ID: WP_000621175.1
• Coordinates: 516148..516510 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU542_RS02640
• Protein ID: WP_000948331.1
• Coordinates: 515981..516151 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU542_RS02610 (511018)	511018..511578	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU542_RS02615 (511786)	511786..512265	+	480	WP_001287088.1	hypothetical protein	-
KU542_RS02620 (512258)	512258..513841	+	1584	WP_001294626.1	PH domain-containing protein	-
KU542_RS02625 (513828)	513828..514319	+	492	WP_001205910.1	PH domain-containing protein	-
KU542_RS02630 (514323)	514323..514682	+	360	WP_000581200.1	holo-ACP synthase	-
KU542_RS02635 (514748)	514748..515896	+	1149	WP_001281145.1	alanine racemase	-
KU542_RS02640 (515981)	515981..516151	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU542_RS02645 (516148)	516148..516510	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU542_RS02650 (516860)	516860..517861	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU542_RS02655 (517980)	517980..518306	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU542_RS02660 (518308)	518308..518787	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU542_RS02665 (518762)	518762..519532	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208436 WP_000621175.1 NZ_CP077893:516148-516510 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208436 NZ_CP103645:c609564-609100 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208436 WP_000948331.1 NZ_CP077893:515981-516151 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208436 NZ_CP103645:c609899-609564 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1