Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2496685..2497199 Replicon chromosome
Accession NZ_CP077889
Organism Staphylococcus aureus strain 333

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU539_RS12280 Protein ID WP_000581792.1
Coordinates 2496685..2496894 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU539_RS12285 Protein ID WP_000138932.1
Coordinates 2496906..2497199 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU539_RS12260 (2492193) 2492193..2493551 - 1359 WP_000611462.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
KU539_RS12265 (2493566) 2493566..2494636 - 1071 WP_000159633.1 D-alanine--D-alanine ligase -
KU539_RS12270 (2494954) 2494954..2496156 + 1203 WP_001109935.1 FtsW/RodA/SpoVE family cell cycle protein -
KU539_RS12275 (2496420) 2496420..2496557 - 138 WP_000828354.1 Lmo0850 family protein -
KU539_RS12280 (2496685) 2496685..2496894 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU539_RS12285 (2496906) 2496906..2497199 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU539_RS12290 (2497365) 2497365..2498849 + 1485 WP_000571549.1 cardiolipin synthase -
KU539_RS12295 (2498877) 2498877..2499524 + 648 WP_001187629.1 HD domain-containing protein -
KU539_RS12300 (2500337) 2500337..2501209 - 873 WP_000725802.1 membrane protein insertase YidC -
KU539_RS12305 (2501295) 2501295..2501936 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208421 WP_000581792.1 NZ_CP077889:c2496894-2496685 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208421 NZ_CP103639:2672224-2672709 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCACAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACTCGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTTTCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTGTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTACGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACGATTCTATTTACACCACGGATTCAAAGCCTCAACCACCCAGGAACGCACTCTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208421 WP_000138932.1 NZ_CP077889:c2497199-2496906 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208421 NZ_CP103639:2671970-2672236 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCACGGACCGACTTTATTCTTGAGATGGCTTGCCAGGCGGCAGAAAACGTCATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACTCCGCTCTCGATAAATTGCTG
GCGAGAAAACCACAATGGGAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References