Detailed information of TA system TA208402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 308443..308972
• Replicon: chromosome
• Accession: NZ_CP077888
• Organism: Staphylococcus aureus strain 334

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU519_RS01820
• Protein ID: WP_000621175.1
• Coordinates: 308443..308805 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU519_RS01825
• Protein ID: WP_000948331.1
• Coordinates: 308802..308972 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU519_RS01800 (305421)	305421..306191	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
KU519_RS01805 (306166)	306166..306645	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KU519_RS01810 (306647)	306647..306973	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU519_RS01815 (307092)	307092..308093	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU519_RS01820 (308443)	308443..308805	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU519_RS01825 (308802)	308802..308972	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU519_RS01830 (309057)	309057..310205	-	1149	WP_001281145.1	alanine racemase	-
KU519_RS01835 (310271)	310271..310630	-	360	WP_000581200.1	holo-ACP synthase	-
KU519_RS01840 (310634)	310634..311125	-	492	WP_001205910.1	PH domain-containing protein	-
KU519_RS01845 (311112)	311112..312695	-	1584	WP_001294626.1	PH domain-containing protein	-
KU519_RS01850 (312688)	312688..313167	-	480	WP_001287088.1	hypothetical protein	-
KU519_RS01855 (313375)	313375..313935	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208402 WP_000621175.1 NZ_CP077888:c308805-308443 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208402 NZ_CP103633:c4991302-4990991 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208402 WP_000948331.1 NZ_CP077888:c308972-308802 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208402 NZ_CP103633:c4990990-4990700 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1