Detailed information of TA system TA208388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1866699..1867228
• Replicon: chromosome
• Accession: NZ_CP077885
• Organism: Staphylococcus aureus strain 355

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU507_RS09605
• Protein ID: WP_000621175.1
• Coordinates: 1866699..1867061 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU507_RS09610
• Protein ID: WP_000948331.1
• Coordinates: 1867058..1867228 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU507_RS09585 (1863678)	1863678..1864448	-	771	WP_225806156.1	RNA polymerase sigma factor SigB	-
KU507_RS09590 (1864423)	1864423..1864902	-	480	WP_225806155.1	anti-sigma B factor RsbW	-
KU507_RS09595 (1864904)	1864904..1865230	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU507_RS09600 (1865349)	1865349..1866350	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU507_RS09605 (1866699)	1866699..1867061	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU507_RS09610 (1867058)	1867058..1867228	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU507_RS09615 (1867313)	1867313..1868461	-	1149	WP_001281145.1	alanine racemase	-
KU507_RS09620 (1868527)	1868527..1868886	-	360	WP_000581200.1	holo-ACP synthase	-
KU507_RS09625 (1868890)	1868890..1869381	-	492	WP_001205910.1	PH domain-containing protein	-
KU507_RS09630 (1869368)	1869368..1870951	-	1584	WP_001294626.1	PH domain-containing protein	-
KU507_RS09635 (1870944)	1870944..1871423	-	480	WP_001287088.1	hypothetical protein	-
KU507_RS09640 (1871632)	1871632..1872192	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208388 WP_000621175.1 NZ_CP077885:c1867061-1866699 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208388 NZ_CP103633:2477716-2478003 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208388 WP_000948331.1 NZ_CP077885:c1867228-1867058 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208388 NZ_CP103633:2477477-2477716 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1