Detailed information of TA system TA020838

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4923618..4924453
• Replicon: chromosome
• Accession: NC_004431
• Organism: Escherichia coli CFT073

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VR61
• Locus tag: C_RS24365
• Protein ID: WP_000854747.1
• Coordinates: 4923618..4923995 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C_RS24370
• Protein ID: WP_024182258.1
• Coordinates: 4924085..4924453 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C_RS24335	4919569..4919910	-	342	Protein_4608	DUF4942 domain-containing protein	-
C_RS24340	4920004..4921503	+	1500	WP_000174402.1	IS21-like element ISEc10 family transposase	-
C_RS24345	4921500..4922255	+	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
C_RS24350	4922321..4922839	-	519	Protein_4611	DUF4942 domain-containing protein	-
C_RS24355	4922924..4923121	-	198	WP_000839291.1	DUF957 domain-containing protein	-
C_RS24360	4923133..4923621	-	489	WP_000761667.1	hypothetical protein	-
C_RS24365	4923618..4923995	-	378	WP_000854747.1	TA system toxin CbtA family protein	Toxin
C_RS24370	4924085..4924453	-	369	WP_024182258.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C_RS24375	4924532..4924753	-	222	WP_000692312.1	DUF987 domain-containing protein	-
C_RS24380	4924822..4925298	-	477	WP_001186756.1	RadC family protein	-
C_RS24385	4925313..4925798	-	486	WP_000860054.1	antirestriction protein	-
C_RS24390	4925889..4926707	-	819	WP_001175140.1	DUF945 domain-containing protein	-
C_RS28070	4926797..4927030	-	234	WP_001278280.1	DUF905 family protein	-
C_RS24395	4927036..4927713	-	678	WP_001097307.1	hypothetical protein	-
C_RS24400	4927861..4928541	-	681	WP_001282928.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14095.03 Da
• Isoelectric Point: 6.8524

>T20838 WP_000854747.1 NC_004431:c4923995-4923618 [Escherichia coli CFT073]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNDITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T20838 NC_004431:c4923995-4923618 [Escherichia coli CFT073]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACCCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13497.32 Da
• Isoelectric Point: 6.7344

>AT20838 WP_024182258.1 NC_004431:c4924453-4924085 [Escherichia coli CFT073]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLGQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGCVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT20838 NC_004431:c4924453-4924085 [Escherichia coli CFT073]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCATCTGGGCCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGCTG
TGGTTGCGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H2VFZ1