Detailed information of TA system TA208373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 412819..413348
• Replicon: chromosome
• Accession: NZ_CP077880
• Organism: Staphylococcus aureus strain 356

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU497_RS02145
• Protein ID: WP_000621175.1
• Coordinates: 412986..413348 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU497_RS02140
• Protein ID: WP_000948331.1
• Coordinates: 412819..412989 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU497_RS02110 (407855)	407855..408415	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU497_RS02115 (408624)	408624..409103	+	480	WP_001287088.1	hypothetical protein	-
KU497_RS02120 (409096)	409096..410679	+	1584	WP_001294626.1	PH domain-containing protein	-
KU497_RS02125 (410666)	410666..411157	+	492	WP_001205910.1	PH domain-containing protein	-
KU497_RS02130 (411161)	411161..411520	+	360	WP_000581200.1	holo-ACP synthase	-
KU497_RS02135 (411586)	411586..412734	+	1149	WP_001281145.1	alanine racemase	-
KU497_RS02140 (412819)	412819..412989	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU497_RS02145 (412986)	412986..413348	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU497_RS02150 (413697)	413697..414698	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU497_RS02155 (414817)	414817..415143	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU497_RS02160 (415145)	415145..415624	+	480	WP_225806155.1	anti-sigma B factor RsbW	-
KU497_RS02165 (415599)	415599..416369	+	771	WP_225806156.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208373 WP_000621175.1 NZ_CP077880:412986-413348 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208373 NZ_CP103627:82939-83337 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208373 WP_000948331.1 NZ_CP077880:412819-412989 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208373 NZ_CP103627:82712-82939 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1