Detailed information of TA system TA208363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2844488..2845264
• Replicon: chromosome
• Accession: NZ_CP077877
• Organism: Staphylococcus aureus strain 357

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: KU522_RS14175
• Protein ID: WP_000031108.1
• Coordinates: 2844488..2844640 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: KU522_RS14180
• Protein ID: WP_001251224.1
• Coordinates: 2844665..2845264 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU522_RS14160 (2840451)	2840451..2841272	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
KU522_RS14165 (2841735)	2841735..2843120	-	1386	WP_000116224.1	class II fumarate hydratase	-
KU522_RS14170 (2843316)	2843316..2843711	-	396	WP_000901021.1	hypothetical protein	-
KU522_RS14175 (2844488)	2844488..2844640	-	153	WP_000031108.1	SAS053 family protein	Toxin
KU522_RS14180 (2844665)	2844665..2845264	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
KU522_RS14185 (2845423)	2845423..2845893	-	471	WP_050956565.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
KU522_RS14190 (2845898)	2845898..2847025	-	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
KU522_RS14195 (2847176)	2847176..2847898	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
KU522_RS14200 (2847891)	2847891..2849348	-	1458	WP_000649907.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T208363 WP_000031108.1 NZ_CP077877:c2844640-2844488 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T208363 NZ_CP103626:1857104-1857358 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT208363 WP_001251224.1 NZ_CP077877:c2845264-2844665 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT208363 NZ_CP103626:1856856-1857107 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4