Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 171190..171704 Replicon chromosome
Accession NZ_CP077877
Organism Staphylococcus aureus strain 357

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU522_RS00850 Protein ID WP_000581792.1
Coordinates 171190..171399 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU522_RS00855 Protein ID WP_000138932.1
Coordinates 171411..171704 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU522_RS00830 (166698) 166698..168056 - 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
KU522_RS00835 (168071) 168071..169141 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
KU522_RS00840 (169459) 169459..170661 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU522_RS00845 (170925) 170925..171062 - 138 WP_000828354.1 Lmo0850 family protein -
KU522_RS00850 (171190) 171190..171399 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU522_RS00855 (171411) 171411..171704 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU522_RS00860 (171870) 171870..173354 + 1485 WP_050956558.1 cardiolipin synthase -
KU522_RS00865 (173382) 173382..174029 + 648 WP_001187629.1 HD domain-containing protein -
KU522_RS00870 (174842) 174842..175714 - 873 WP_000725802.1 membrane protein insertase YidC -
KU522_RS00875 (175800) 175800..176441 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208351 WP_000581792.1 NZ_CP077877:c171399-171190 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208351 NZ_CP103623:c4350064-4349891 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208351 WP_000138932.1 NZ_CP077877:c171704-171411 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208351 NZ_CP103623:c4350388-4350092 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References