Detailed information of TA system TA208350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 151433..151962
• Replicon: chromosome
• Accession: NZ_CP077877
• Organism: Staphylococcus aureus strain 357

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU522_RS00760
• Protein ID: WP_000621175.1
• Coordinates: 151433..151795 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU522_RS00765
• Protein ID: WP_000948331.1
• Coordinates: 151792..151962 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU522_RS00740 (148412)	148412..149182	-	771	WP_225806156.1	RNA polymerase sigma factor SigB	-
KU522_RS00745 (149157)	149157..149636	-	480	WP_225806155.1	anti-sigma B factor RsbW	-
KU522_RS00750 (149638)	149638..149964	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU522_RS00755 (150083)	150083..151084	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU522_RS00760 (151433)	151433..151795	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU522_RS00765 (151792)	151792..151962	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU522_RS00770 (152047)	152047..153195	-	1149	WP_001281145.1	alanine racemase	-
KU522_RS00775 (153261)	153261..153620	-	360	WP_000581200.1	holo-ACP synthase	-
KU522_RS00780 (153624)	153624..154115	-	492	WP_001205910.1	PH domain-containing protein	-
KU522_RS00785 (154102)	154102..155685	-	1584	WP_001294626.1	PH domain-containing protein	-
KU522_RS00790 (155678)	155678..156157	-	480	WP_001287088.1	hypothetical protein	-
KU522_RS00795 (156366)	156366..156926	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208350 WP_000621175.1 NZ_CP077877:c151795-151433 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208350 NZ_CP103623:4337475-4337852 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208350 WP_000948331.1 NZ_CP077877:c151962-151792 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208350 NZ_CP103623:4337054-4337428 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1