Detailed information of TA system TA020835

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4339604..4340436
• Replicon: chromosome
• Accession: NC_004431
• Organism: Escherichia coli CFT073

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2VCF5
• Locus tag: C_RS21565
• Protein ID: WP_000854681.1
• Coordinates: 4340059..4340436 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3Z9KHH3
• Locus tag: C_RS21560
• Protein ID: WP_021562928.1
• Coordinates: 4339604..4339969 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C_RS21525	4335521..4336201	+	681	WP_001278650.1	WYL domain-containing protein	-
C_RS21530	4336349..4337026	+	678	WP_001097301.1	hypothetical protein	-
C_RS21535	4337032..4337265	+	234	WP_001278287.1	DUF905 family protein	-
C_RS21540	4337355..4338173	+	819	WP_001175163.1	DUF945 domain-containing protein	-
C_RS21545	4338265..4338750	+	486	WP_000206666.1	antirestriction protein	-
C_RS21550	4338765..4339241	+	477	WP_001186171.1	RadC family protein	-
C_RS21555	4339304..4339525	+	222	WP_000692300.1	DUF987 domain-containing protein	-
C_RS21560	4339604..4339969	+	366	WP_021562928.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C_RS21565	4340059..4340436	+	378	WP_000854681.1	TA system toxin CbtA family protein	Toxin
C_RS29535	4340433..4340582	+	150	Protein_4106	hypothetical protein	-
C_RS29540	4340658..4340855	+	198	WP_023563519.1	DUF957 domain-containing protein	-
C_RS21580	4340940..4341782	+	843	WP_001280503.1	DUF4942 domain-containing protein	-
C_RS21590	4342378..4342875	+	498	WP_000509808.1	hypothetical protein	-
C_RS21595	4343042..4343965	+	924	WP_001351210.1	carboxylate/amino acid/amine transporter	-
C_RS21600	4343969..4344787	-	819	WP_000779409.1	lipoprotein NlpA	-
C_RS21610	4345009..4345302	+	294	WP_001297978.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-116)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13963.89 Da
• Isoelectric Point: 7.4348

>T20835 WP_000854681.1 NC_004431:4340059-4340436 [Escherichia coli CFT073]
MKTLPDTHIREASHCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTSHNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T20835 NC_004431:4340059-4340436 [Escherichia coli CFT073]
ATGAAAACATTACCCGACACACACATACGGGAGGCATCGCACTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAGAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCAGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13528.32 Da
• Isoelectric Point: 6.4648

>AT20835 WP_021562928.1 NC_004431:4339604-4339969 [Escherichia coli CFT073]
VSDTLPGTTHPDNNNRPWWGLPCTVMPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSGE
LSPRYQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 366 bp

>AT20835 NC_004431:4339604-4339969 [Escherichia coli CFT073]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACCGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCC
CTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGTTCA
GCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGTGAA
CTCAGCCCTCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCTTCGGCTCCTGTGG
TTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACAAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A0H2VCF5

Antitoxin

• AlphaFold DB: A0A3Z9KHH3