Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2838061..2838575 Replicon chromosome
Accession NZ_CP077872
Organism Staphylococcus aureus strain 358

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU513_RS14280 Protein ID WP_000581792.1
Coordinates 2838366..2838575 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU513_RS14275 Protein ID WP_000138932.1
Coordinates 2838061..2838354 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU513_RS14255 (2833324) 2833324..2833965 + 642 WP_000483153.1 thiamine phosphate synthase -
KU513_RS14260 (2834051) 2834051..2834923 + 873 WP_000725802.1 membrane protein insertase YidC -
KU513_RS14265 (2835736) 2835736..2836383 - 648 WP_001187629.1 HD domain-containing protein -
KU513_RS14270 (2836411) 2836411..2837895 - 1485 WP_050956558.1 cardiolipin synthase -
KU513_RS14275 (2838061) 2838061..2838354 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU513_RS14280 (2838366) 2838366..2838575 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU513_RS14285 (2838703) 2838703..2838840 + 138 WP_000828354.1 Lmo0850 family protein -
KU513_RS14290 (2839104) 2839104..2840306 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU513_RS14295 (2840624) 2840624..2841694 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
KU513_RS14300 (2841709) 2841709..2843067 + 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208349 WP_000581792.1 NZ_CP077872:2838366-2838575 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208349 NZ_CP103623:c4187429-4187112 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208349 WP_000138932.1 NZ_CP077872:2838061-2838354 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208349 NZ_CP103623:c4187674-4187423 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References