Detailed information of TA system TA208326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2173154..2173683
• Replicon: chromosome
• Accession: NZ_CP077870
• Organism: Staphylococcus aureus strain 360

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU523_RS10925
• Protein ID: WP_000621175.1
• Coordinates: 2173154..2173516 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU523_RS10930
• Protein ID: WP_000948331.1
• Coordinates: 2173513..2173683 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU523_RS10905 (2170133)	2170133..2170903	-	771	WP_225806156.1	RNA polymerase sigma factor SigB	-
KU523_RS10910 (2170878)	2170878..2171357	-	480	WP_225806155.1	anti-sigma B factor RsbW	-
KU523_RS10915 (2171359)	2171359..2171685	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU523_RS10920 (2171804)	2171804..2172805	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU523_RS10925 (2173154)	2173154..2173516	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU523_RS10930 (2173513)	2173513..2173683	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU523_RS10935 (2173768)	2173768..2174916	-	1149	WP_001281145.1	alanine racemase	-
KU523_RS10940 (2174982)	2174982..2175341	-	360	WP_000581200.1	holo-ACP synthase	-
KU523_RS10945 (2175345)	2175345..2175836	-	492	WP_001205910.1	PH domain-containing protein	-
KU523_RS10950 (2175823)	2175823..2177406	-	1584	WP_001294626.1	PH domain-containing protein	-
KU523_RS10955 (2177399)	2177399..2177878	-	480	WP_001287088.1	hypothetical protein	-
KU523_RS10960 (2178087)	2178087..2178647	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208326 WP_000621175.1 NZ_CP077870:c2173516-2173154 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208326 NZ_CP103621:c3828034-3827717 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208326 WP_000948331.1 NZ_CP077870:c2173683-2173513 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208326 NZ_CP103621:c3827724-3827437 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGGGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1