Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3337389..3338043 Replicon chromosome
Accession NC_004431
Organism Escherichia coli CFT073

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag C_RS16515 Protein ID WP_000244781.1
Coordinates 3337389..3337796 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C_RS16520 Protein ID WP_000354046.1
Coordinates 3337777..3338043 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C_RS16495 3333346..3335079 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
C_RS16500 3335085..3335795 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C_RS16505 3335820..3336716 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C_RS16510 3336828..3337349 + 522 WP_001055874.1 flavodoxin FldB -
C_RS16515 3337389..3337796 - 408 WP_000244781.1 protein YgfX Toxin
C_RS16520 3337777..3338043 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C_RS16525 3338286..3339266 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
C_RS16530 3339343..3340002 - 660 WP_000250274.1 hemolysin III family protein -
C_RS16535 3340166..3340477 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
C_RS16540 3340522..3341955 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T20832 WP_000244781.1 NC_004431:c3337796-3337389 [Escherichia coli CFT073]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T20832 NC_004431:c3337796-3337389 [Escherichia coli CFT073]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT20832 WP_000354046.1 NC_004431:c3338043-3337777 [Escherichia coli CFT073]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT20832 NC_004431:c3338043-3337777 [Escherichia coli CFT073]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References