Detailed information of TA system TA208312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1849423..1849952
• Replicon: chromosome
• Accession: NZ_CP077869
• Organism: Staphylococcus aureus strain 365

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU517_RS09250
• Protein ID: WP_000621175.1
• Coordinates: 1849423..1849785 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU517_RS09255
• Protein ID: WP_000948331.1
• Coordinates: 1849782..1849952 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU517_RS09230 (1846402)	1846402..1847172	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU517_RS09235 (1847147)	1847147..1847626	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU517_RS09240 (1847628)	1847628..1847954	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU517_RS09245 (1848073)	1848073..1849074	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU517_RS09250 (1849423)	1849423..1849785	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU517_RS09255 (1849782)	1849782..1849952	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU517_RS09260 (1850037)	1850037..1851185	-	1149	WP_001280702.1	alanine racemase	-
KU517_RS09265 (1851251)	1851251..1851610	-	360	WP_000581194.1	holo-ACP synthase	-
KU517_RS09270 (1851614)	1851614..1852105	-	492	WP_001205915.1	PH domain-containing protein	-
KU517_RS09275 (1852092)	1852092..1853675	-	1584	WP_001294642.1	PH domain-containing protein	-
KU517_RS09280 (1853668)	1853668..1854147	-	480	WP_001287083.1	hypothetical protein	-
KU517_RS09285 (1854356)	1854356..1854916	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208312 WP_000621175.1 NZ_CP077869:c1849785-1849423 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208312 NZ_CP103618:c3968674-3968357 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208312 WP_000948331.1 NZ_CP077869:c1849952-1849782 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208312 NZ_CP103618:c3968364-3968077 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1