Detailed information of TA system TA208306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 608552..609029
• Replicon: chromosome
• Accession: NZ_CP077869
• Organism: Staphylococcus aureus strain 365

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: KU517_RS02985
• Protein ID: WP_000052076.1
• Coordinates: 608552..608740 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: KU517_RS02990
• Protein ID: WP_001059690.1
• Coordinates: 608769..609029 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU517_RS02955 (604590)	604590..605060	+	471	WP_000824462.1	hypothetical protein	-
KU517_RS02960 (605401)	605401..606087	+	687	WP_001823812.1	thermonuclease family protein	-
KU517_RS02965 (606444)	606444..606644	+	201	WP_001059082.1	cold-shock protein	-
KU517_RS02970 (607143)	607143..607361	-	219	WP_000005888.1	hypothetical protein	-
KU517_RS02975 (607423)	607423..607707	-	285	WP_001004397.1	hypothetical protein	-
KU517_RS02980 (607795)	607795..608364	-	570	WP_001106657.1	lipoprotein N-acylation protein LnsA	-
KU517_RS02985 (608552)	608552..608740	-	189	WP_000052076.1	hypothetical protein	Toxin
KU517_RS02990 (608769)	608769..609029	-	261	WP_001059690.1	hypothetical protein	Antitoxin
KU517_RS02995 (609370)	609370..609573	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
KU517_RS03000 (609570)	609570..609806	-	237	WP_000368687.1	hypothetical protein	-
KU517_RS03005 (610030)	610030..610611	+	582	WP_001140697.1	histidine phosphatase family protein	-
KU517_RS03010 (610684)	610684..611301	-	618	WP_000255554.1	LysE/ArgO family amino acid transporter	-
KU517_RS03015 (611457)	611457..611951	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
KU517_RS03020 (612350)	612350..612772	-	423	WP_000974455.1	organic hydroperoxide resistance protein	-
KU517_RS03025 (612920)	612920..613636	+	717	WP_000150029.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	selk / selq / seb	603232..636579	33347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T208306 WP_000052076.1 NZ_CP077869:c608740-608552 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T208306 NZ_CP103615:11346-11762 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT208306 WP_001059690.1 NZ_CP077869:c609029-608769 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT208306 NZ_CP103615:11119-11349 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56