Detailed information of TA system TA208289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2351825..2352354
• Replicon: chromosome
• Accession: NZ_CP077867
• Organism: Staphylococcus aureus strain 367

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU500_RS11385
• Protein ID: WP_000621175.1
• Coordinates: 2351992..2352354 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU500_RS11380
• Protein ID: WP_000948331.1
• Coordinates: 2351825..2351995 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU500_RS11350 (2346861)	2346861..2347421	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
KU500_RS11355 (2347630)	2347630..2348109	+	480	WP_001287083.1	hypothetical protein	-
KU500_RS11360 (2348102)	2348102..2349685	+	1584	WP_001294642.1	PH domain-containing protein	-
KU500_RS11365 (2349672)	2349672..2350163	+	492	WP_001205915.1	PH domain-containing protein	-
KU500_RS11370 (2350167)	2350167..2350526	+	360	WP_000581194.1	holo-ACP synthase	-
KU500_RS11375 (2350592)	2350592..2351740	+	1149	WP_001280702.1	alanine racemase	-
KU500_RS11380 (2351825)	2351825..2351995	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU500_RS11385 (2351992)	2351992..2352354	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU500_RS11390 (2352703)	2352703..2353704	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU500_RS11395 (2353823)	2353823..2354149	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU500_RS11400 (2354151)	2354151..2354630	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU500_RS11405 (2354605)	2354605..2355375	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208289 WP_000621175.1 NZ_CP077867:2351992-2352354 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208289 NZ_CP103611:c1117145-1116927 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACACTCGAACGCGT
GATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACCGTATTTTATTCAGCCGCTGACCATCGCCTTGCCGAGC
TGACCATGAATAAACTGTATGACAAAATCCCGCCTTCCGTGTGGAAATTTGTCCGTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208289 WP_000948331.1 NZ_CP077867:2351825-2351995 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208289 NZ_CP103611:c1117547-1117173 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTGAACGATCCAACATCGGCCATCAATTTGCAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1