Detailed information of TA system TA208267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 691119..691648
• Replicon: chromosome
• Accession: NZ_CP077865
• Organism: Staphylococcus aureus strain 370

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU528_RS03535
• Protein ID: WP_000621175.1
• Coordinates: 691119..691481 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU528_RS03540
• Protein ID: WP_000948331.1
• Coordinates: 691478..691648 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU528_RS03515 (688098)	688098..688868	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU528_RS03520 (688843)	688843..689322	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU528_RS03525 (689324)	689324..689650	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU528_RS03530 (689769)	689769..690770	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU528_RS03535 (691119)	691119..691481	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU528_RS03540 (691478)	691478..691648	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU528_RS03545 (691733)	691733..692881	-	1149	WP_001280702.1	alanine racemase	-
KU528_RS03550 (692947)	692947..693306	-	360	WP_000581194.1	holo-ACP synthase	-
KU528_RS03555 (693310)	693310..693801	-	492	WP_001205915.1	PH domain-containing protein	-
KU528_RS03560 (693788)	693788..695371	-	1584	WP_001294642.1	PH domain-containing protein	-
KU528_RS03565 (695364)	695364..695843	-	480	WP_001287083.1	hypothetical protein	-
KU528_RS03570 (696052)	696052..696612	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208267 WP_000621175.1 NZ_CP077865:c691481-691119 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208267 NZ_CP103609:1449907-1450209 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCTCGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208267 WP_000948331.1 NZ_CP077865:c691648-691478 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208267 NZ_CP103609:1450245-1450586 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1