Detailed information of TA system TA208258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2195124..2195653
• Replicon: chromosome
• Accession: NZ_CP077863
• Organism: Staphylococcus aureus strain 371

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU524_RS10515
• Protein ID: WP_000621175.1
• Coordinates: 2195291..2195653 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU524_RS10510
• Protein ID: WP_000948331.1
• Coordinates: 2195124..2195294 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU524_RS10480 (2190160)	2190160..2190720	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
KU524_RS10485 (2190929)	2190929..2191408	+	480	WP_001287083.1	hypothetical protein	-
KU524_RS10490 (2191401)	2191401..2192984	+	1584	WP_001294642.1	PH domain-containing protein	-
KU524_RS10495 (2192971)	2192971..2193462	+	492	WP_001205915.1	PH domain-containing protein	-
KU524_RS10500 (2193466)	2193466..2193825	+	360	WP_000581194.1	holo-ACP synthase	-
KU524_RS10505 (2193891)	2193891..2195039	+	1149	WP_001280702.1	alanine racemase	-
KU524_RS10510 (2195124)	2195124..2195294	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU524_RS10515 (2195291)	2195291..2195653	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU524_RS10520 (2196002)	2196002..2197003	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU524_RS10525 (2197122)	2197122..2197448	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU524_RS10530 (2197450)	2197450..2197929	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU524_RS10535 (2197904)	2197904..2198674	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208258 WP_000621175.1 NZ_CP077863:2195291-2195653 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208258 NZ_CP103605:c5151372-5150989 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208258 WP_000948331.1 NZ_CP077863:2195124-2195294 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208258 NZ_CP103605:c5151614-5151372 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1